SLC2A2 ELISA Kit

Catalog No. ABIN5674769

Product Details SLC2A2 ELISA Kit

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.33 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for SLC2A2

Target: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Alternative Name: Glucose Transporter 2 (GLUT2) (SLC2A2 Products)

Synonyms: glut2 ELISA Kit, wu:fb62h10 ELISA Kit, slc2a2 ELISA Kit, MGC83262 ELISA Kit, SLC2A2 ELISA Kit, GLUT2 ELISA Kit, GTT2 ELISA Kit, Glut2 ELISA Kit, AI266973 ELISA Kit, Glut-2 ELISA Kit, solute carrier family 2 (facilitated glucose transporter), member 2 ELISA Kit, solute carrier family 2 member 2 L homeolog ELISA Kit, solute carrier family 2 member 2 ELISA Kit, solute carrier family 2, facilitated glucose transporter member 2 ELISA Kit, slc2a2 ELISA Kit, slc2a2.L ELISA Kit, SLC2A2 ELISA Kit, LOC100534484 ELISA Kit, Slc2a2 ELISA Kit

Background: Alternative name: SLC2A2, SLC2-A2, Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver

UniProt: P12336

Pathways: Warburg Effect