Aromatase ELISA Kit

Catalog No. ABIN5674814

Product Details Aromatase ELISA Kit

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0117 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for Aromatase

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Alternative Name: Aromatase (ARO) (CYP19A1 Products)

Synonyms: ARO ELISA Kit, ARO1 ELISA Kit, CPV1 ELISA Kit, CYAR ELISA Kit, CYP19 ELISA Kit, CYPXIX ELISA Kit, P-450AROM ELISA Kit, Aromatase ELISA Kit, Cyp19 ELISA Kit, Cyp19a ELISA Kit, p450arom ELISA Kit, P450 arom ELISA Kit, aro ELISA Kit, cyp19 ELISA Kit, cyp19a ELISA Kit, p450arom-A ELISA Kit, P450AROM ELISA Kit, Ar ELISA Kit, ArKO ELISA Kit, Int-5 ELISA Kit, Int5 ELISA Kit, CYP19P1 ELISA Kit, CYP19A1 ELISA Kit, CYP19a1 ELISA Kit, P450aromA ELISA Kit, ar1 ELISA Kit, cypXIX ELISA Kit, etID309862.18 ELISA Kit, zgc:136541 ELISA Kit, AroB ELISA Kit, CYP19a2 ELISA Kit, P450aromB ELISA Kit, cyp19b ELISA Kit, zgc:92614 ELISA Kit, cyp19a1 ELISA Kit, cytochrome P450 family 19 subfamily A member 1 ELISA Kit, cytochrome P450, family 19, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450 family 19 subfamily A member 1 L homeolog ELISA Kit, aromatase ELISA Kit, cytochrome P450 aromatase ELISA Kit, cytochrome P450, family 19, subfamily A, polypeptide 1 ELISA Kit, cytochrome P450 19A1 ELISA Kit, cytochrome P450, family 17, subfamily A, polypeptide 1 ELISA Kit, cytochrome P450, family 19 ELISA Kit, cytochrome P450, family 19, subfamily A, polypeptide 1a ELISA Kit, cytochrome P450, family 19, subfamily A, polypeptide 1b ELISA Kit, cytochrome P450 19A1-like ELISA Kit, CYP19A1 ELISA Kit, Cyp19a1 ELISA Kit, cyp19a1.L ELISA Kit, SAV_2383 ELISA Kit, pfcyp19b-tstr ELISA Kit, CYP19 ELISA Kit, cyp19a1a ELISA Kit, cyp19a1b ELISA Kit, LOC101174651 ELISA Kit

Background: Alternative name: CYP19A1, CYPXIX, ARO, ARO1, CPV1, CYAR, CYP19, P450AROM, P450-AROM, Estrogen synthase, Cytochrome P450,Family 19,Subfamily A,Polypeptide 1

Gene ID: 13075

UniProt: P28649

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis