TSPO ELISA Kit

Catalog No. ABIN5674815

Product Details TSPO ELISA Kit

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0113 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Alternative Name: Translocator Protein (TSPO) (TSPO Products)

Synonyms: LOC732875 ELISA Kit, LOC100136652 ELISA Kit, LOC100280949 ELISA Kit, BZRP ELISA Kit, TSPO ELISA Kit, Bzrp ELISA Kit, IBP ELISA Kit, PBR ELISA Kit, Tspo1 ELISA Kit, MBR ELISA Kit, PTBZR02 ELISA Kit, Ptbzr ELISA Kit, RATPTBZR02 ELISA Kit, BPBS ELISA Kit, DBI ELISA Kit, PBS ELISA Kit, PKBS ELISA Kit, PTBR ELISA Kit, mDRC ELISA Kit, pk18 ELISA Kit, peripheral-type benzodiazepine receptor ELISA Kit, Peripheral-type benzodiazepine receptor ELISA Kit, putative peripheral-type benzodiazepine receptor ELISA Kit, translocator protein ELISA Kit, translocator protein homolog ELISA Kit, hypothetical protein ELISA Kit, LOC732875 ELISA Kit, MAV_3896 ELISA Kit, xccb100_2086 ELISA Kit, Smp_102510 ELISA Kit, Sros_1911 ELISA Kit, tspo ELISA Kit, LOC100280949 ELISA Kit, PGTG_16395 ELISA Kit, TSPO ELISA Kit, Tspo ELISA Kit

Background: Alternative name: IBP, PKBS, PBR, pk18, mDRC, MBR, BZRP, DBI, PTBR, Mitochondrial benzodiazepine receptor, Peripheral-Type Benzodiazepine Receptor/Recognition Site

Gene ID: 12257

UniProt: P50637

Pathways: Cellular Response to Molecule of Bacterial Origin