Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CYP27B1 ELISA Kit

CYP27B1 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5674843
  • Target See all CYP27B1 ELISA Kits
    CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))
    Reactivity
    • 3
    • 3
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0.29 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Featured
    Discover our best selling CYP27B1 ELISA Kit
    Top Product
    Discover our top product CYP27B1 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all CYP27B1 ELISA Kits
    CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))
    Alternative Name
    Cytochrome P450 27B1 (CYP27B1) (CYP27B1 Products)
    Synonyms
    cp2b ELISA Kit, cyp1 ELISA Kit, cyp1alpha ELISA Kit, cyp27b ELISA Kit, p450c1 ELISA Kit, pddr ELISA Kit, vdd1 ELISA Kit, vddr ELISA Kit, vddri ELISA Kit, vdr ELISA Kit, CP2B ELISA Kit, CYP1 ELISA Kit, CYP1alpha ELISA Kit, CYP27B ELISA Kit, P450c1 ELISA Kit, PDDR ELISA Kit, VDD1 ELISA Kit, VDDR ELISA Kit, VDDRI ELISA Kit, VDR ELISA Kit, Cyp40 ELISA Kit, Cp2b ELISA Kit, Cyp1 ELISA Kit, Cyp27b ELISA Kit, Pddr ELISA Kit, Vdd1 ELISA Kit, Vddr ELISA Kit, VddrI ELISA Kit, Vdr ELISA Kit, cytochrome P450 family 27 subfamily B member 1 ELISA Kit, cytochrome P450, family 27, subfamily B, polypeptide 1 ELISA Kit, cytochrome P450, family 27, subfamily b, polypeptide 1 ELISA Kit, cyp27b1 ELISA Kit, CYP27B1 ELISA Kit, Cyp27b1 ELISA Kit
    Background
    Alternative name: VDD1, PDDR, CYP1, P450c1, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase, Cytochrome P450 Family 27 Subfamily B Polypeptide 1, Calcidiol 1-monooxygenase, VD3 1A hydroxylase
    Gene ID
    1594
    UniProt
    O15528
    Pathways
    Metabolism of Steroid Hormones and Vitamin D
You are here:
Support