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c-MYC ELISA Kit

MYC Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Catalog No. ABIN5690750
  • Target See all c-MYC (MYC) ELISA Kits
    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
    Reactivity
    • 10
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    DNA-Binding ELISA
    Application
    ELISA
    Purpose
    Human c-Myc Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical c-Myc binding sequences to semi-quantitatively detect active c-Myc in lysates or nuclear extracts.
    Sample Type
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificity
    The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.
    Characteristics
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Components
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all c-MYC (MYC) ELISA Kits
    c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
    Alternative Name
    c-Myc (MYC Products)
    Synonyms
    MRTL ELISA Kit, MYCC ELISA Kit, bHLHe39 ELISA Kit, c-Myc ELISA Kit, CMYC ELISA Kit, c-myc ELISA Kit, C-MYC ELISA Kit, LOC100136746 ELISA Kit, ARC ELISA Kit, B430311C09Rik ELISA Kit, MYC ELISA Kit, NOP ELISA Kit, Nop30 ELISA Kit, RNCMYC ELISA Kit, mMyc ELISA Kit, AU016757 ELISA Kit, Myc2 ELISA Kit, Niard ELISA Kit, Nird ELISA Kit, cmyc ELISA Kit, zc-myc ELISA Kit, c-myc II ELISA Kit, myc-B ELISA Kit, myc2 ELISA Kit, myc1 ELISA Kit, CMyc ELISA Kit, zgc:55680 ELISA Kit, CG10798 ELISA Kit, D-Myc ELISA Kit, DM ELISA Kit, DMYc ELISA Kit, Dm ELISA Kit, Dmel\CG10798 ELISA Kit, Dmyc ELISA Kit, EG:BACN5I9.1 ELISA Kit, anon-WO03040301.171 ELISA Kit, bHLHe57 ELISA Kit, c-MYC ELISA Kit, d-myc ELISA Kit, dMYC ELISA Kit, dMyc ELISA Kit, dMyc1 ELISA Kit, da ELISA Kit, dm ELISA Kit, dm/dMyc ELISA Kit, dm/myc ELISA Kit, dmyc ELISA Kit, dmyc1 ELISA Kit, l(1)G0139 ELISA Kit, l(1)G0354 ELISA Kit, l(1)G0359 ELISA Kit, myc ELISA Kit, MYC proto-oncogene, bHLH transcription factor ELISA Kit, v-myc myelocytomatosis viral oncogene homolog ELISA Kit, nucleolar protein 3 (apoptosis repressor with CARD domain) ELISA Kit, myelocytomatosis oncogene ELISA Kit, MYC proto-oncogene, bHLH transcription factor a ELISA Kit, MYC proto-oncogene, bHLH transcription factor L homeolog ELISA Kit, MYC proto-oncogene, bHLH transcription factor S homeolog ELISA Kit, v-myc myelocytomatosis viral oncogene homolog (avian) ELISA Kit, MYC proto-oncogene, bHLH transcription factor b ELISA Kit, CG10798 gene product from transcript CG10798-RB ELISA Kit, MYC ELISA Kit, LOC100136746 ELISA Kit, Nol3 ELISA Kit, Myc ELISA Kit, myca ELISA Kit, myc.L ELISA Kit, myc.S ELISA Kit, mycb ELISA Kit
    Gene ID
    4609
    UniProt
    P01106
    Pathways
    p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
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