LTBP2 ELISA Kit

Catalog No. ABIN5690903

Product Details LTBP2 ELISA Kit

Target: LTBP2 (Latent Transforming Growth Factor beta Binding Protein 2 (LTBP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Latent TGF-beta bp2/LTBP-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Latent TGF-beta bp2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for LTBP2

Target: LTBP2 (Latent Transforming Growth Factor beta Binding Protein 2 (LTBP2))

Alternative Name: Latent TGF-beta bp2 (LTBP-2 (LTBP2 Products)

Synonyms: C14orf141 ELISA Kit, GLC3D ELISA Kit, LTBP3 ELISA Kit, MSPKA ELISA Kit, MSTP031 ELISA Kit, WMS3 ELISA Kit, AW208642 ELISA Kit, LTBP2 ELISA Kit, latent transforming growth factor beta binding protein 2 ELISA Kit, latent transforming growth factor beta binding protein 3 ELISA Kit, LTBP2 ELISA Kit, Ltbp2 ELISA Kit, LTBP3 ELISA Kit, ltbp2 ELISA Kit

Pathways: Growth Factor Binding