Growth Hormone 1 ELISA Kit

Catalog No. ABIN577092

Product Details Growth Hormone 1 ELISA Kit

Target: Growth Hormone 1 (GH1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: This hGH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to hGH. Standards or samples are then added to the microtiter plate wells and incubated. After wash all wells, hGH if present, will bind to the antibody pre-coated on the wells. In order to quantitatively determine the amount of hGH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific to hGH is added to each well to “sandwich” the hGH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3', 5,5' Tetramethyl-benzidene) substrate solution is added to each well. This enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hGH and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Analytical Method: Quantitative

Sensitivity: The minimal detectable concentration of hGH by this assay is estimated to be 0.5ng/mL.

Components: Standards: 1 set/2 vials

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Target Details for Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Abstract: GH1 Products

Synonyms: GH ELISA Kit, GH-N ELISA Kit, GHN ELISA Kit, IGHD1B ELISA Kit, hGH-N ELISA Kit, Gh1 ELISA Kit, GH1 ELISA Kit, gh ELISA Kit, Gh ELISA Kit, GH2 ELISA Kit, RNGHGP ELISA Kit, ghl ELISA Kit, gh-n ELISA Kit, ghn ELISA Kit, ighd1b ELISA Kit, gh1 ELISA Kit, ghb-A ELISA Kit, GHI ELISA Kit, GHB3 ELISA Kit, growth hormone 1 ELISA Kit, growth hormone ELISA Kit, somatotropin-like ELISA Kit, somatotropin ELISA Kit, growth hormone prepeptide ELISA Kit, growth hormone 1 L homeolog ELISA Kit, growth hormone 1 S homeolog ELISA Kit, Somatotropin-1 ELISA Kit, GH1 ELISA Kit, Gh ELISA Kit, GH ELISA Kit, LOC100305005 ELISA Kit, Gh1 ELISA Kit, LOC100534452 ELISA Kit, LOC100232594 ELISA Kit, LOC100303681 ELISA Kit, gh1 ELISA Kit, LOC100356068 ELISA Kit, LOC100136588 ELISA Kit, gh1.L ELISA Kit, gh1.S ELISA Kit, LOC109081196 ELISA Kit

Target Type: Hormone

Background: Human growth hormone (GH) is a 22kDa monomeric protein produced and stored in somatotrophs in the anterior pituitary gland. GH is released from the pituitary into the bloodstream in a pulsatile manner under the regulatory control of hypothalamic somatostatin (SS) and GH-releasing factor (GHRF) [1]. The timing and frequency of GH release appears to be regulated by somatostatin, while the amplitude of GH release is determined by GHRF. A minor fraction (~10%) of GH in circulation exists in a smaller 20 kDa form [2]. GH has profound effects on tissue growth and metabolism, which is thought to be mediated through GH-dependent production of IGF-I and IGF-II, and their associated binding proteins. GH apparently stimulates IGF production after binding to specific cell surface receptors in the liver and, possibly, other tissues. Almost 50% of GH in circulation is bound to a high affinity GH binding protein (GHBP), which represents the extracellular domain of the GH receptor. Deficient GH secretion can occur in a number of clinical conditions [3]. However, evaluation of GH deficiency is complicated by the episodic nature of GH secretion and low circulating levels. A variety of physiologic and pharmacologic stimuli have been used to stimulate pituitary GH release during testing and failure to achieve a normal serum GH level in response to at least 2 GH stimulation or provocative tests is considered to be a diagnostic of GH deficiency [4]. The definition of a normal serum GH response is controversial, although published values generally range from 5 to 10 ng/mL. GH excess (or acromegaly) can be caused either by direct GH hypersecretion or GH excess secondary to GHRF hypersecretion.

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process