Inhibitory Subunit of NF-KappaB epsilon ELISA Kit
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- Target
- Inhibitory Subunit of NF-KappaB epsilon
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Human IkB-epsilon/NF-kappa-BIE ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Human IkB-epsilon.
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Expiry Date
- 6 months
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- Target
- Inhibitory Subunit of NF-KappaB epsilon
- Alternative Name
- IkB-epsilon (NF-kappa-BIE)
- Synonyms
- IKBE ELISA Kit, NFKB inhibitor epsilon ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon ELISA Kit, NFKBIE ELISA Kit, Nfkbie ELISA Kit
- Gene ID
- 4794
- UniProt
- O00221
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