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ACVRL1 ELISA Kit
ACVRL1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
0.41-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details ACVRL1 ELISA Kit
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Target
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ACVRL1
(Activin A Receptor Type II-Like 1 (ACVRL1))
Reactivity
All reactivities for ACVRL1 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.41-100 ng/mL
Minimum Detection Limit
0.41 ng/mL
Application
ELISA
Purpose
Mouse ALK-1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for ACVRL1
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Target
See all ACVRL1 ELISA Kits
ACVRL1
(Activin A Receptor Type II-Like 1 (ACVRL1))
Alternative Name
ALK-1 (ACVRL1 Products )
Synonyms
ACVRL1 ELISA Kit, vgb ELISA Kit, alk1 ELISA Kit, zehn1109 ELISA Kit, hm:zehn1109 ELISA Kit, ACVRLK1 ELISA Kit, ALK-1 ELISA Kit, ALK1 ELISA Kit, HHT ELISA Kit, HHT2 ELISA Kit, ORW2 ELISA Kit, SKR3 ELISA Kit, TSR-I ELISA Kit, R-3 ELISA Kit, R3 ELISA Kit, SETHKIR ELISA Kit, AI115505 ELISA Kit, AI427544 ELISA Kit, Acvrlk1 ELISA Kit, Alk1 ELISA Kit, activin A receptor like type 1 ELISA Kit, activin A receptor type II-like 1 ELISA Kit, activin A receptor, type II-like 1 ELISA Kit, ACVRL1 ELISA Kit, acvrl1 ELISA Kit, Acvrl1 ELISA Kit
Gene ID
20568
UniProt
P97430
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