IL-1 beta ELISA Kit

Catalog No. ABIN6719816

Supplier Product No.: koa0211

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-1 beta. There is 1 publication available.

Quick Overview for IL-1 beta ELISA Kit (ABIN6719816)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5 pg/mL - 800 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details IL-1 beta ELISA Kit

Minimum Detection Limit: 12.5 pg/mL

Supplier Product No.: koa0211

Supplier: Rockland

Purpose: Mouse IL-1 beta Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse IL-1 beta. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 12.5 pg/mL-800 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 800pg/mL, 400pg/mL, 200pg/mL, 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL mouse IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse IL-1 beta standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: V118-S269

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for IL-1 beta ELISA Kit (ABIN6719816)

Zhang, Wang, Wu, Liu, Zhu, Zhu, Xing: "Humanized anti‑TLR4 monoclonal antibody ameliorates lipopolysaccharide‑related acute kidney injury by inhibiting TLR4/NF‑κB signaling." in: Molecular medicine reports, Vol. 24, Issue 2, (2021) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1 beta

Background:

Synonyms: Catabolin, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL-1 beta, IL1, IL1 BETA, IL1B, IL1B_HUMAN, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, Interleukin-1 beta, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta

Background: Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Gene ID: 16176

NCBI Accession: NP_032387

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome