Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1) ELISA Kit
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- Target
- Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 156 pg/mL - 10000 pg/mL
- Minimum Detection Limit
- 156 pg/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA), Serum
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: FLT, FLT 1, Flt-1, FLT1, Fms like tyrosine kinase 1, Fms related tyrosine kinase 1, Fms-like tyrosine kinase 1, Tyrosine protein kinase FRT, Tyrosine protein kinase receptor FLT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular endothelial growth factor receptor 1, Vascular endothelial growth factor/vascular permeability factor receptor, Vascular permeability factor receptor, VEGFR 1, VEGFR-1, VEGFR1, VGFR1_HUMAN
Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
Gene Name: FLT1
Production: Natural and recombinant human Svegfr1. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: sf21, Immunogen sequence: S27-H687
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Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1) ELISA Kit
Reactivity: Human Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human sVEGFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human sVEGFR1 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Sodium EDTA
ELISA Dilution: 156pg/mL-10000pg/mL
Other Performance Data: DOE: 2-8° C - 03/27/19 -20° C - 09/25/19
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Target
- Soluble Vascular Endothelial Growth Factor Receptor 1(sFlt-1/sVEGFR-1)
- Alternative Name
- sVEGFR1 - sFLT1
- Synonyms
- AI323757 ELISA Kit, Flt-1 ELISA Kit, VEGFR-1 ELISA Kit, VEGFR1 ELISA Kit, sFlt1 ELISA Kit, FMS-like tyrosine kinase 1 ELISA Kit, Flt1 ELISA Kit
- Gene ID
- 2321
- NCBI Accession
- NP_001153392
- UniProt
- P17948
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