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SERPINB10 ELISA Kit
SERPINB10
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details SERPINB10 ELISA Kit
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Target
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SERPINB10
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 10 (SERPINB10))
Reactivity
All reactivities for SERPINB10 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse Serpinb10 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse Serpinb10.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SERPINB10
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Target
See all SERPINB10 ELISA Kits
SERPINB10
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 10 (SERPINB10))
Alternative Name
Serpinb10 (SERPINB10 Products )
Synonyms
PI-10 ELISA Kit, PI10 ELISA Kit, 9830131G07 ELISA Kit, BB233602 ELISA Kit, Serpinb10-ps ELISA Kit, Trespin ELISA Kit, MENT-1 ELISA Kit, SERPINB10 ELISA Kit, pl10 ELISA Kit, bomapin ELISA Kit, serpin family B member 10 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 10 ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 10 B ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 10 L homeolog ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 10 ELISA Kit, heterochromatin-associated protein MENT-like ELISA Kit, SERPINB10 ELISA Kit, Serpinb10 ELISA Kit, SERPINB10B ELISA Kit, serpinb10.L ELISA Kit, LOC100228737 ELISA Kit
UniProt
Q8K1K6
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