HIST2H2BE ELISA Kit

Catalog No. ABIN6730714

Product Details HIST2H2BE ELISA Kit

Target: HIST2H2BE (Histone H2B Type 2-E (HIST2H2BE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human HIST2H2BE ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human HIST2H2BE.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for HIST2H2BE

Target: HIST2H2BE (Histone H2B Type 2-E (HIST2H2BE))

Alternative Name: HIST2H2BE (HIST2H2BE Products)

Synonyms: RGD1562346 ELISA Kit, AV127319 ELISA Kit, H2b-613 ELISA Kit, T25626 ELISA Kit, DKFZp469C0528 ELISA Kit, H2b ELISA Kit, GL105 ELISA Kit, H2B ELISA Kit, H2B.1 ELISA Kit, H2BFQ ELISA Kit, H2BGL105 ELISA Kit, H2BQ ELISA Kit, histone cluster 2 H2B family member E ELISA Kit, histone cluster 2, H2be ELISA Kit, histone cluster 1, H2bl ELISA Kit, histone cluster 2 H2B family member e ELISA Kit, histone H2B ELISA Kit, Hist2h2be ELISA Kit, HIST2H2BE ELISA Kit, Hist1h2bl ELISA Kit, H2B ELISA Kit

UniProt: Q16778