KHDRBS1 ELISA Kit

Catalog No. ABIN6951166

Product Details KHDRBS1 ELISA Kit

Target: KHDRBS1 (KH Domain Containing, RNA Binding, Signal Transduction Associated 1 (KHDRBS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human P62 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for KHDRBS1

Target: KHDRBS1 (KH Domain Containing, RNA Binding, Signal Transduction Associated 1 (KHDRBS1))

Alternative Name: P62 (KHDRBS1 Products)

Synonyms: Sam68 ELISA Kit, p62 ELISA Kit, p68 ELISA Kit, fj90g10 ELISA Kit, khdrbs1 ELISA Kit, wu:fa18g12 ELISA Kit, wu:fa56c01 ELISA Kit, wu:fc91b01 ELISA Kit, wu:fj90g10 ELISA Kit, zgc:113899 ELISA Kit, KHDRBS1 ELISA Kit, P62 ELISA Kit, khdrbs1l ELISA Kit, sb:cb97 ELISA Kit, wu:fb07d11 ELISA Kit, wu:fi43c05 ELISA Kit, zgc:85948 ELISA Kit, KH RNA binding domain containing, signal transduction associated 1 ELISA Kit, KH domain containing, RNA binding, signal transduction associated 1a ELISA Kit, KH domain containing, RNA binding, signal transduction associated 1 ELISA Kit, KH domain containing, RNA binding, signal transduction associated 1 S homeolog ELISA Kit, KH domain containing, RNA binding, signal transduction associated 1b ELISA Kit, KHDRBS1 ELISA Kit, khdrbs1a ELISA Kit, khdrbs1 ELISA Kit, Khdrbs1 ELISA Kit, khdrbs1.S ELISA Kit, khdrbs1b ELISA Kit

Gene ID: 8878

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy