ATP6V1F ELISA Kit

Catalog No. ABIN6951273

Product Details ATP6V1F ELISA Kit

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ATP6V1F ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ATP6V1F

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Alternative Name: ATP6V1F (ATP6V1F Products)

Synonyms: Atp6s14 ELISA Kit, Vma7 ELISA Kit, atp6s14 ELISA Kit, atp6v1f ELISA Kit, vatf ELISA Kit, ATP6S14 ELISA Kit, VATF ELISA Kit, 1110004G16Rik ELISA Kit, ATPase H+ transporting V1 subunit F ELISA Kit, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F S homeolog ELISA Kit, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F L homeolog ELISA Kit, ATPase, H+ transporting, lysosomal V1 subunit F ELISA Kit, Atp6v1f ELISA Kit, atp6v1f.S ELISA Kit, ATP6V1F ELISA Kit, atp6v1f.L ELISA Kit

Gene ID: 9296

UniProt: Q16864

Pathways: Transition Metal Ion Homeostasis, Proton Transport