SEMA3F ELISA Kit
SEMA3F
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all SEMA3F ELISA Kits
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SEMA3F
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))
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Reactivity
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Mouse
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Mouse SEMA3F ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product SEMA3F ELISA Kit
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Application Notes
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Optimal working dilution should be determined by the investigator.
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Expiry Date
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6 months
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Target
See all SEMA3F ELISA Kits
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SEMA3F
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))
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Alternative Name
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SEMA3F (SEMA3F Products)
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Synonyms
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Sema4 ELISA Kit, Semak ELISA Kit, SemaIV ELISA Kit, semaphorin-3F ELISA Kit, sema4 ELISA Kit, semak ELISA Kit, sema-iv ELISA Kit, SEMA3F ELISA Kit, SEMA-IV ELISA Kit, SEMA4 ELISA Kit, SEMAK ELISA Kit, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F ELISA Kit, semaphorin 3F ELISA Kit, semaphorin 3F L homeolog ELISA Kit, Sema3f ELISA Kit, SEMA3F ELISA Kit, sema3f ELISA Kit, sema3f.L ELISA Kit
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Gene ID
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20350
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UniProt
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O88632
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Pathways
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Regulation of Cell Size
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