SPAM1 ELISA Kit
SPAM1
Reactivity: Cow
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all SPAM1 ELISA Kits
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SPAM1
(Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
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Reactivity
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Cow
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Bovine SPAM1 ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Application Notes
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Optimal working dilution should be determined by the investigator.
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Expiry Date
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6 months
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Target
See all SPAM1 ELISA Kits
-
SPAM1
(Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
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Alternative Name
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SPAM1 (SPAM1 Products)
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Synonyms
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AV039194 ELISA Kit, Ph-20 ELISA Kit, PH-20 ELISA Kit, PH20 ELISA Kit, HYA1 ELISA Kit, HYAL1 ELISA Kit, HYAL3 ELISA Kit, HYAL5 ELISA Kit, SPAG15 ELISA Kit, Spam ELISA Kit, XEH1 ELISA Kit, spam1 ELISA Kit, im:6902904 ELISA Kit, zgc:158668 ELISA Kit, sperm adhesion molecule 1 ELISA Kit, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) L homeolog ELISA Kit, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit, Spam1 ELISA Kit, SPAM1 ELISA Kit, spam1.L ELISA Kit, spam1 ELISA Kit
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UniProt
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Q2YDK3
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