eIF4EBP1 ELISA Kit

Catalog No. ABIN7830388

Human eIF4EBP1 ELISA Kit, Colorimetric assay for quantification of Human eIF4EBP1.

Quick Overview for eIF4EBP1 ELISA Kit (ABIN7830388)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details eIF4EBP1 ELISA Kit

Purpose: EIF4EBP1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EIF4EBP1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: EIF4EBP1

Background:

Synonyms: 4E-BP1|4EBP1|BP-1|MGC4316|PHAS-I

Gene Description: eukaryotic translation initiation factor 4E binding protein 1

Gene ID: 1978

UniProt: Q13541

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling