BAD ELISA Kit

Catalog No. ABIN7830435

The Human BAD ELISA Kit (ABIN7830435) is a Colorimetric ELISA Kit designed to quantify Human BAD.

Quick Overview for BAD ELISA Kit (ABIN7830435)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details BAD ELISA Kit

Purpose: BAD (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BAD expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD

Background:

Synonyms: BBC2|BCL2L8

Gene Description: BCL2-associated agonist of cell death

Gene ID: 572

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events