HRAS ELISA Kit

Catalog No. ABIN7830972

The Human HRAS ELISA Kit (ABIN7830972) is a Colorimetric ELISA Kit designed to quantify Human HRAS.

Quick Overview for HRAS ELISA Kit (ABIN7830972)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Product Details HRAS ELISA Kit

Purpose: HRAS/KRAS/NRAS (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HRAS/KRAS/NRAS expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS

Background:

Synonyms: C-BAS/HAS|C-H-RAS|C-HA-RAS1|CTLO|H-RASIDX|HAMSV|HRAS1|K-RAS|N-RAS|RASH1

Gene Description: v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Gene ID: 3265

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling