p21 ELISA Kit
Quick Overview for p21 ELISA Kit (ABIN8077766)
Target
See all p21 (CDKN1A) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- p21 Cip1 Colorimetric Cell-Based ELISA Kit for detecting p21 Cip1 in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA Kit
CDKN1A Reactivity: Rat Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A Reactivity: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A Reactivity: Cow Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) ELISA KitCDKN1A Reactivity: Rat Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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Alternative Name
- p21 Cip1
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Background
- Gene Aliases: CAP20, CDK-interacting protein 1, CDKN1, CDKN1A, CDN1A, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation associated protein, Melanoma differentiation associated protein 6, p21, PIC1, SDI1, WAF1
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Gene ID
- 1026
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UniProt
- P38936
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Pathways
- p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
Target See all p21 (CDKN1A) ELISA Kits
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