HMGB1 ELISA Kit
Quick Overview for HMGB1 ELISA Kit (ABIN8080631)
Target
See all HMGB1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 31.2 pg/mL
-
Purpose
- Rat High Mobility Group Protein 1 (HMGB1) ELISA Kit for detecting HMGB1 in serum, plasma, tissue homogenates
-
Analytical Method
- Quantitative
-
Sensitivity
- 12.2 pg/mL
-
Components
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
Material not included
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
High Mobility Group Box 1 (HMGB1) ELISA Kit
HMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Rat Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Dog Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Dog Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Comment
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
Sample Volume
- 100 μL
-
Assay Time
- 3 h
-
Plate
- Pre-coated
-
Assay Precision
-
Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- 4°C for one month, Long term: see manual.
-
Expiry Date
- 12 months
-
-
- HMGB1 (High Mobility Group Box 1 (HMGB1))
-
Alternative Name
- High Mobility Group Protein 1
-
Background
- Gene Aliases: HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein
-
Gene ID
- 25459
-
UniProt
- P63159
-
Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Target See all HMGB1 ELISA Kits
-
