HMGB1 ELISA Kit
Quick Overview for HMGB1 ELISA Kit (ABIN8080635)
Target
See all HMGB1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 31.25 pg/mL
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Purpose
- Human HMGB-1 (High Mobility Group Protein B1) ELISA Kit for detecting HMGB-1 in serum, plasma
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Analytical Method
- Quantitative
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Sensitivity
- 18.75 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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High Mobility Group Box 1 (HMGB1) ELISA Kit
HMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Rat Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Dog Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Dog Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 3 - 4 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 2-8°C for 12 months, Long term: see manual.
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Expiry Date
- 12 months
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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Alternative Name
- HMGB-1
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Background
- Gene Aliases: HMGB1, HMG1, HMG3, SBP-1, Amphoterin
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UniProt
- P09429
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Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Target See all HMGB1 ELISA Kits
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