TGFB1 CLIA Kit

Catalog No. ABIN8088183

The Pig TGFB1 ELISA Kit (ABIN8088183) is a Chemiluminescent ELISA Kit designed to quantify Pig TGFB1.

Quick Overview for TGFB1 CLIA Kit (ABIN8088183)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Pig

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 13.7 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details TGFB1 CLIA Kit

Minimum Detection Limit: 13.7 pg/mL

Purpose: Pig Transforming Growth Factor Beta 1 (TGFb1) CLIA Kit for detecting TGFb1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensitivity: 5.7 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: Transforming Growth Factor Beta 1

Background: Gene Aliases: TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide

UniProt: P07200

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints