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TNF alpha ELISA Kit

Mouse TNF alpha ELISA Kit, Colorimetric assay for quantification of Mouse TNF alpha.
Catalog No. ABIN8088571
$594.35
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for TNF alpha ELISA Kit (ABIN8088571)

Target

See all TNF alpha ELISA Kits
TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity

  • 40
  • 37
  • 32
  • 15
  • 14
  • 13
  • 7
  • 6
  • 4
  • 4
  • 4
  • 4
  • 3
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  • 1
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  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

16 pg/mL - 2000 pg/mL

Application

ELISA

Sample Type

Plasma, Red Blood Cell Lysate, Serum
  • Minimum Detection Limit

    16 pg/mL

    Purpose

    TNF-alpha (Murine) Sandwich ELISA Kit for detecting TNF-alpha in plasma, serum, lysate.

    Analytical Method

    Quantitative

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Assay Time

    4 - 5 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    6 months
  • Target See all TNF alpha ELISA Kits

    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

    Alternative Name

    TNF-alpha

    Background

    Gene Aliases: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2

    Gene ID

    21926

    UniProt

    P06804

    Pathways

    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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