IL-6 ELISA Kit

Catalog No. ABIN8090743

Human IL-6 ELISA Kit, Colorimetric assay for quantification of Human IL-6.

Quick Overview for IL-6 ELISA Kit (ABIN8090743)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.078 pg/mL - 5 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Grade: High Sensitivity

Product Details IL-6 ELISA Kit

Minimum Detection Limit: 0.078 pg/mL

Purpose: Human HS-IL-6 (High sensitive Interleukin 6) high sensitive Accquant ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with IL-6 , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy human serum/plasma (EDTA, Citrate , heparin) (n=10) 1/2 dilution 1.2-6pg/mL Antigen-positive serum of COVID-19 patients (n=5) 1/300- 1/1000 dilution 132-610pg/mL Antigen-positive plasma (EDTA) of COVID-19 patients (n=12) 1/300-1/2000 dilution 185-996pg/mL Healthy human saliva (n=9) 1/2 dilution ND-12pg/mL Human peripheral blood mononuclear cells (2 x 10^6 cells/mL) were cultured with 5 %FBS+ 1640 + double antibody +10 μg PHA/mL, and the cell culture supernatant was detected after 48 hours 1/10000 dilution 5.2 ng/mL 2. KO sample validation (Detect IL-6 KO A549 cells): Sample Type Dilution Ratio Content Wild A549 cells were stimulated with 20 ng/mL(h.IL-11b) for 24 hours to detect cell culture supernatant 1/3000 dilution 1.56 ng/mL KO A549 cells were stimulated with 20 ng/mL(h.IL-11b) for 24 hours to detect cell culture supernatants 1/2 dilution ND KO A549 cells were treated with 20 ng/mL(H.IL-11b) for 24 hours and then cultured with 5 μg/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 2.51 mg/mL) to detect. 1/2 dilution ND Note:ND is lower than the sensitivity of the kit and was not detected 3. Capture antibody and detection antibody by WB KO validation (Detect IL-6 KO A549 cells):

Sensitivity: 0.046 pg/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Assay Time: 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background: Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome