IL12 ELISA Kit

Catalog No. ABIN8126256

This Colorimetric ELISA kit is designed for the quantitative measurement of Dog IL12.

Quick Overview for IL12 ELISA Kit (ABIN8126256)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Dog

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL12 ELISA Kit

Minimum Detection Limit: 15.625 pg/mL

Purpose: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensitivity: 9.375 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Canine IL-12/p70 concentrations in serum, plasma. Detection Range: 15.625 - 1000 pg/mL Sensitivity: < 9.375 pg/mL

Application Details

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-canine IL-12/p70 antibody was pre-coated into 96-well plates. Biotin conjugated anti-canine IL-12/p70 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the canine IL-12/p70 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Canine IL-12/p70 can be calculated using the standard curve.

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12/p70 (Interleukin 12/p70)

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome