Plate | Pre-coated |
Reagent Preparation |
Bring all kit components and samples to room temperature (18-25°C) before use. Dispense 10 µL of lysis buffer solution into 100 µL specimens, mix and stand for one hour (The proportion of lysis buffer and specimens shall be no less than 1:10). (NOTE: This step is required when the sample is cell culture fluid & body fluid & tissue homogenate, If the sample is serum or blood plasma, then this step should be skipped.) Wash Solution Dilute 10 mL of Wash Solution concentrate (100X) with 990 mL of de-ionized or distilled water to prepare 1,000 mL of Wash Solution (1X). |
Assay Procedure |
It is recommended that all Calibrators and Samples be added in duplicate to the Microtiter Plate. |
Calculation of Results |
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Restrictions | For Research Use only |
Storage | 4 °C |
Storage Comment | All reagents provided are stored at 4°C. Refer to the expiration date on the label. |
Expiry Date | The expiry date is stated on the label. |