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Nuclear Factor kappa B p50 (NFkB p50) ELISA Kit

Reactivity: Human Colorimetric DNA-Binding ELISA
Catalog No. ABIN965406
  • Target
    Nuclear Factor kappa B p50 (NFkB p50)
    Reactivity
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    DNA-Binding ELISA
    Application
    ELISA
    Purpose
    NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
    Analytical Method
    Quantitative
    Characteristics
    A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate. NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).
  • Application Notes
    NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).
    Comment

    Detection Kit Type: ELISA Kit

    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
  • Target
    Nuclear Factor kappa B p50 (NFkB p50)
    Alternative Name
    NF kB (p50)
    Synonyms
    NF-KB1 ELISA Kit, NF-kappaB ELISA Kit, NF-kappaB1 ELISA Kit, p105 ELISA Kit, p50 ELISA Kit, p50/p105 ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 ELISA Kit, Nfkb1 ELISA Kit
    Background
    The NF-kB/Rel family of transcription factors is comprised of several structurally related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), c-Rel/NF-kB [1]. Members of this family are responsible for regulating over 150 target genes, including the expression of inflammatory cytokines, chemokines, immunoreceptors and cell adhesion molecules. Because of this, NF-kB has often been called a ‘central mediator of the human immune response' [2]. Acting as dimers, these transcription factors bind to DNA sequences, collectively called kB, sites thereby regulating expression of target genes. In most cells, Rel/ NF-kB transcription complexes are present in an inactive form in the cytoplasm, bound to an inhibitor IB. Certain stimuli result in the phosphorylation, ubiquitination and subsequent degradation of IkB proteins thereby enabling translocation of NF-kB into the nucleus [3]. The most common Rel/NF-kB dimmer in mammals contains p50-RelA (p50/p65) heterodimers and is specifically called NF-kB. One of the target genes activated by NF-kB is that encoding IkBα. This feedback mechanism allows newly-synthesized IkBα to enter the nucleus, remove NF-kB from DNA and transport it back to the cytoplasm thereby restoring its inactive state. The importance of Rel/NF-kB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutics [4-6].
    Synonyms: NF-kB Transcription Factor Kit, p50 kit, EIA kit, NF kappaB ELISA Assay
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