DCX HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
DCX
WB, IP
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Human
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- Target See all Doublecortin (DCX) products
- Doublecortin (DCX)
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
DCX HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-DCX full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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DCX 293T Cell Transient Overexpression Lysate(Denatured)
DCX WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-DCX antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- Doublecortin (DCX)
- Alternative Name
- DCX (DCX Products)
- Synonyms
- DCX Lysate, DBCN Lysate, DC Lysate, LISX Lysate, SCLH Lysate, XLIS Lysate, Dbct Lysate, 18C15.5 Lysate, doublecortin Lysate, DCX Lysate, Dcx Lysate
- Background
-
Full Gene Name: doublecortin
Synonyms: DBCN,DC,LISX,SCLH,XLIS - Gene ID
- 1641
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