FES HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- Target See all FES products
- FES (Feline Sarcoma Oncogene (FES))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
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FES HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-FES full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-FES antibody by Western Blots.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- Target
- FES (Feline Sarcoma Oncogene (FES))
- Alternative Name
- FES (FES Products)
- Synonyms
- FPS Lysate, RGD1564385 Lysate, AI586313 Lysate, BB137047 Lysate, c-fes Lysate, FES Lysate, c-fps Lysate, FES proto-oncogene, tyrosine kinase Lysate, feline sarcoma oncogene Lysate, FES Lysate, Fes Lysate, fes Lysate
- Background
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Full Gene Name: feline sarcoma oncogene
Synonyms: FPS - Gene ID
- 2242
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