anti-Human BCL2 antibody for Immunohistochemistry (Frozen Sections)

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 41-54
247
78
64
61
40
28
28
26
22
21
16
15
15
15
11
11
9
9
9
9
8
7
7
7
7
6
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1257
273
251
70
36
21
21
19
6
4
3
3
3
2
2
Host
Mouse
701
633
19
6
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
62
57
53
50
43
25
24
24
23
15
14
14
14
14
14
14
13
13
11
11
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1028
474
473
424
409
406
224
187
155
155
81
42
33
15
13
9
6
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN3044481
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4283388 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283367 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN1105504 IHC (fro) IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see BL-2 2
1 ABIN4283369 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
1 ABIN1491491 ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 AA 41-54 Log in to see BL-2 0
1 ABIN957155 IHC IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal 0
1 ABIN957159 IHC IHC (fro) IHC (p) IP WB Rabbit AA 61-76 Log in to see Polyclonal 0
1 ABIN4283406 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN4283394 ELISA ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see 8C8 0
1 ABIN1105507 IHC (fro) IHC (p) Rabbit IgG Log in to see E17 6
1 ABIN781707 IHC (fro) FACS IF IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl2-100 0
1 ABIN126981 IHC (fro) IP WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN4283436 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 41-54 Log in to see SPM117 0
1 ABIN4283446 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 41-54 Log in to see 100-D5-124 0
1 ABIN4283433 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 41-54 Log in to see SPM530 0
1 ABIN4283443 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 41-54 Log in to see 124 0
1 ABIN609189 ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see 0
1 ABIN4283414 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 100-D5 0
1 ABIN4283425 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see 100-D5 0
1 ABIN4283502 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG AA 41-54 Log in to see 100-D5-124 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(247), (78), (64), (61), (40), (28), (28), (26), (22), (21), (16), (15), (15), (15), (11), (11), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1257), (273), (251), (70), (36), (21), (21), (19), (6), (4), (3), (3), (3), (2), (2)
Host Mouse
(701), (633), (19), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(62), (57), (53), (50), (43), (25), (24), (24), (23), (15), (14), (14), (14), (14), (14), (14), (13), (13), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1028), (474), (473), (424), (409), (406), (224), (187), (155), (155), (81), (42), (33), (15), (13), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 91 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN3044481) Images
Purpose Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2 (BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2 (BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Gene Name: B-cell CLL/lymphoma 2
Protein Name: Apoptosis regulator Bcl-2
Purification Ascites
Immunogen Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.
Clone Bcl-2-100
Isotype IgG1
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN3044481) Images back to top
Antigen
Alternative Name BCL2 (BCL2 Antibody Abstract)
Background Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14,18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Synonyms: PPP1R50|protein phosphatase 1, regulatory subunit 50|ONCOGENE B-CELL LEUKEMIA 2
Gene ID 596, 12043, 24224
UniProt P10415, P10417, P49950
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN3044481) Images back to top
Application Notes WB: Concentration: 1-2 μg/mL, Species: Human
IHC-P: Concentration: 0.4-1 μg/mL, Species: Human
IHC-F: Concentration: 0.4-1 μg/mL, Species: Human
ICC: Concentration: 1 μg/mL, Species: Human
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN3044481) Images back to top
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-BCL2 antibody (ABIN3044481)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, 2015

Xu, Liu, Zhang, Wang, Wang, Yang, Huo, Sun: "Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy." in: Neural regeneration research, Vol. 7, Issue 17, pp. 1318-24, 2015

Ding, Wang, Zhao, Sun, Zhai: "Protective Effects of Baicalin on A?????-Induced Learning and Memory Deficit, Oxidative Stress, and Apoptosis in Rat." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 623-32, 2015

Yang, Li, Liu, Shi, Zhang: "Inhibitory effect of tetramethylpyrazine preconditioning on overload training-induced myocardial apoptosis in rats." in: Chinese journal of integrative medicine, Vol. 21, Issue 6, pp. 423-30, 2015

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, 2015

Li, Wang, Wang: "Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina." in: Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB), Vol. 31, Issue 4, pp. 352-9, 2015

Zhan, Hu, Yi, An, Huang: "Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4142-8, 2015

Peng, Chen, Chen, Fang, Cui, Zuo, Deng, Chen, Geng, Lai: "Aflatoxin B1 affects apoptosis and expression of Bax, Bcl-2, and Caspase-3 in thymus and bursa of fabricius in broiler chickens." in: Environmental toxicology, 2015

Tang, Zhang, Qi, Chen, Li, Liu, He, Li, Zu: "Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line." in: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1851-1856, 2015

Liu, Su, Zhang, Zhang, Wang, Qian: "Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-?-induced protein 8-like-2 in septic rats." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 386-98, 2015

Huang, Lin, Su, Ye: "Forsythiaside protects against hydrogen peroxide-induced oxidative stress and apoptosis in PC12 cell." in: Neurochemical research, Vol. 40, Issue 1, pp. 27-35, 2015

Yin, Li, Li, Yu, Cai, Zhang, Wang, Zhang, Zhou, Cheng, Gao: "Critical role of prohibitin in endothelial cell apoptosis caused by glycated low-density lipoproteins and protective effects of grape seed procyanidin B2." in: Journal of cardiovascular pharmacology, Vol. 65, Issue 1, pp. 13-21, 2015

Liu, Zheng, Wang, Han, Yuan, Zhao: "Transplantation of olfactory ensheathing cells attenuates acute carbon monoxide poisoning-induced brain damages in rats." in: Neurochemical research, Vol. 40, Issue 1, pp. 70-80, 2015

Wang, Zhang, Chai, Liu, Berk: "The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression." in: BMC psychiatry, Vol. 14, pp. 349, 2015

Wu, Dong, Li: "Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload." in: International journal of molecular medicine, Vol. 35, Issue 4, pp. 893-900, 2015

Sun, Zhang, Guan, Zhou, Sun: "Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice." in: International journal of clinical and experimental medicine, Vol. 8, Issue 1, pp. 358-67, 2015

Han, Yu, Song, Luo, Wu: "Agmatine protects Müller cells from high-concentration glucose-induced cell damage via N-methyl-D-aspartic acid receptor inhibition." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 1098-106, 2015

Gao, Xie, Peng, Han, Jiang, Han, Wang: "Hispidulin inhibits proliferation and enhances chemosensitivity of gallbladder cancer cells by targeting HIF-1?." in: Experimental cell research, Vol. 332, Issue 2, pp. 236-46, 2015

Lu, Zheng, Lai, Li: "Xuezhikang () reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family." in: Chinese journal of integrative medicine, 2015

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, 2014

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN3044481) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN3044481) Anti-Bcl-2 antibody (monoclonal), IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN3044481) Anti-Bcl-2 antibody (monoclonal), Western blotting Lane 1: Rat Heart Tissue Lysate La...
Immunohistochemistry (IHC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN3044481) Anti-Bcl-2 antibody (monoclonal), IHC(P) IHC(P): Human Mammary Cancer Tissue