anti-Human BCL2 antibody for Immunohistochemistry (Frozen Sections)

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 41-54
246
80
64
44
40
29
28
27
21
17
15
15
15
11
10
10
10
9
9
9
8
7
7
7
7
6
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1237
267
254
68
38
21
19
17
6
4
3
3
3
2
2
Host
Mouse
667
641
19
6
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
61
55
48
46
36
23
21
21
18
11
11
11
11
10
10
10
10
10
10
9
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
987
442
441
441
381
356
210
155
142
124
91
42
33
15
13
9
3
3
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN3044481
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.252602 ABIN1689470 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see BL-2 0
8.5 ABIN549103 IHC (fro) IP WB Rabbit Log in to see Polyclonal 1
7 ABIN1491491 ICC IHC IHC (fro) IHC (p) WB Mouse IgG1 AA 41-54 Log in to see BL-2 0
4 ABIN1105504 IHC (fro) IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see BL-2 2
4 ABIN957155 IHC IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal 0
4 ABIN957159 IHC IHC (fro) IHC (p) IP WB Rabbit AA 61-76 Log in to see Polyclonal 0
4 ABIN126981 IHC (fro) IP WB Rabbit N-Term Log in to see Polyclonal 0
4 ABIN2477599 IHC (fro) FACS IHC (p) IP WB Mouse IgG1 Log in to see 1E2 4
4 ABIN3030101 ICC IHC (fro) IHC (p) WB Mouse IgG1 AA 41-54 Log in to see Bcl-2-100-aka BL-2 0
4 ABIN187919 IHC (fro) WB Rabbit N-Term Log in to see Polyclonal 0
4 ABIN537389 IHC (fro) IHC (p) IP Rabbit Log in to see Polyclonal 0
1 ABIN1105507 IHC (fro) IHC (p) Rabbit IgG Log in to see E17 6
1 ABIN781707 IHC (fro) FACS IF IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl2-100 0
1 ABIN609189 ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see 0
1 ABIN957156 IHC IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal 0
1 ABIN1976983 FACS ICC IHC IHC (fro) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl2-100 0
1 ABIN609190 IHC IHC (fro) IHC (p) Mouse IgG1, kappa Log in to see 124 0
1 ABIN2589984 IHC IHC (fro) IHC (p) Mouse IgG1, kappa N-Term Log in to see BCL2-A4 0
1 ABIN1838360 IHC IHC (fro) IHC (p) Rabbit IgG Log in to see E17 0
1 ABIN5648834 FACS ICC IHC (fro) IHC (p) IP WB FITC Mouse IgG1 Log in to see Bcl2-100 0

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(246), (80), (64), (44), (40), (29), (28), (27), (21), (17), (15), (15), (15), (11), (10), (10), (10), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1237), (267), (254), (68), (38), (21), (19), (17), (6), (4), (3), (3), (3), (2), (2)
Host Mouse
(667), (641), (19), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(61), (55), (48), (46), (36), (23), (21), (21), (18), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(987), (442), (441), (441), (381), (356), (210), (155), (142), (124), (91), (42), (33), (15), (13), (9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 145 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN3044481) Images
Purpose Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2 (BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2 (BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Gene Name: B-cell CLL/lymphoma 2
Protein Name: Apoptosis regulator Bcl-2
Purification Ascites
Immunogen Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.
Clone Bcl-2-100
Isotype IgG1
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN3044481) Images back to top
Antigen
Alternative Name BCL2 (BCL2 Antibody Abstract)
Background Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14,18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Synonyms: PPP1R50|protein phosphatase 1, regulatory subunit 50|ONCOGENE B-CELL LEUKEMIA 2
Gene ID 596, 12043, 24224
UniProt P10415, P10417, P49950
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN3044481) Images back to top
Application Notes WB: Concentration: 1-2 μg/mL, Species: Human
IHC-P: Concentration: 0.4-1 μg/mL, Species: Human
IHC-F: Concentration: 0.4-1 μg/mL, Species: Human
ICC: Concentration: 1 μg/mL, Species: Human
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN3044481) Images back to top
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-BCL2 antibody (ABIN3044481)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, 2018

Feng, Yan, Zhou, Liang, Liang, Zhao, Dong, Ling: "Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis." in: Oncotarget, Vol. 7, Issue 40, pp. 64575-64588, 2018

Guo, Lin, Shao, Rong, Zhang: "BMP‑7 suppresses excessive scar formation by activating the BMP‑7/Smad1/5/8 signaling pathway." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1957-1963, 2018

Chen, Zhu, Yang, Wei, Chen, He, Ji: "Ailanthone induces G2/M cell cycle arrest and apoptosis of SGC‑7901 human gastric cancer cells." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 6821-6827, 2018

Liang, Zhong, Gong, Wang, Zhu, Liu, Yang et al.: "Fibroblast growth factor 21 protects rat cardiomyocytes from endoplasmic reticulum stress by promoting the fibroblast growth factor receptor 1-extracellular signal‑regulated kinase 1/2 signaling ..." in: International journal of molecular medicine, Vol. 40, Issue 5, pp. 1477-1485, 2018

Bai, Yang, Luo: "Effects of 5-hydroxy-4'-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells." in: Oncology letters, Vol. 15, Issue 4, pp. 5227-5235, 2018

Yang, Shi, Soomro, Hu, Du, She: "Hepatitis E Virus Induces Hepatocyte Apoptosis via Mitochondrial Pathway in Mongolian Gerbils." in: Frontiers in microbiology, Vol. 9, pp. 460, 2018

Wang, Li, Yang, Gao, Lin, Wang, Zhou, Hu: "Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer's disease induced by Aβ1-40." in: American journal of translational research, Vol. 10, Issue 3, pp. 796-805, 2018

Li, Wang, Zhou, Shuang, Zhao, Chen: "Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 2384-2390, 2018

Yang, Wu, Luo, Zhang: "CCAAT/enhancer binding protein homologous protein knockdown alleviates hypoxia-induced myocardial injury in rat cardiomyocytes exposed to high glucose." in: Experimental and therapeutic medicine, Vol. 15, Issue 5, pp. 4213-4222, 2018

Qi, Xue, Lv, Sun, Du, Cai, Li, Gu, Wang: "Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway." in: Oncology letters, Vol. 15, Issue 5, pp. 6400-6408, 2018

Zhou, Wang, Xu, Wan, Qu, Fan, Chen, Sun, Liu: "Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 14, pp. 18620-30, 2017

Hu, Sun, Zhang, Zhang, Hu: "Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway." in: Bioscience reports, Vol. 36, Issue 3, 2017

Guo, Wang, Gao, Zhang, Chen, Xu, Hu, Jing, Jing, Li, Wang, Zhu: "Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 22, pp. 3951-3960, 2017

Hu, Lv, Zhang, Meng: "Comparative Study on the Protective Effects of Salidroside and Hypoxic Preconditioning for Attenuating Anoxia-Induced Apoptosis in Pheochromocytoma (PC12) Cells." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 22, pp. 4082-4091, 2017

Ustarroz-Cano, Garcia-Pelaez, Cervantes-Yepez, Lopez-Valdez, Fortoul: "Thymic cytoarchitecture changes in mice exposed to vanadium." in: Journal of immunotoxicology, Vol. 14, Issue 1, pp. 9-14, 2017

Xu, Tan, Jiang, Chen, Lai, Zhang, Liang: "Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside-induced premature ovarian failure." in: Chinese medicine, Vol. 12, pp. 10, 2017

Guo, Liu: "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 2030-2036, 2017

Xu, Deng, Wang, Chen, Li, Fan: "RKTG overexpression inhibits proliferation and induces apoptosis of human leukemia cells via suppression of the ERK and PI3K/AKT signaling pathways." in: Oncology letters, Vol. 14, Issue 1, pp. 965-970, 2017

Ma, Liu, Song, Tian, Wei, Wang, Li, Yang: "Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells." in: Oncology letters, Vol. 14, Issue 2, pp. 1512-1518, 2017

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN3044481) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN3044481) Anti-Bcl-2 antibody (monoclonal), IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN3044481) Anti-Bcl-2 antibody (monoclonal), Western blotting Lane 1: Rat Heart Tissue Lysate La...
Immunohistochemistry (IHC) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN3044481) Anti-Bcl-2 antibody (monoclonal), IHC(P) IHC(P): Human Mammary Cancer Tissue