anti-Human BCL2 antibody for Western Blotting

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
AA 1-211
251
79
64
45
40
29
28
27
20
17
15
15
15
10
10
10
10
9
9
9
8
7
7
7
7
6
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1222
265
242
70
38
21
19
19
6
4
3
3
3
2
2
Host
Rabbit
644
643
18
6
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
60
54
47
46
36
23
21
20
18
11
11
11
11
10
10
10
10
10
10
10
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Western Blotting (WB)
973
446
445
442
379
357
206
155
148
125
81
42
33
15
13
9
6
3
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN3042814
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.08196 ABIN388095 IF IHC (p) WB Rabbit Ig Fraction AA 75-110, BH3 Domain Log in to see Polyclonal 19
3.08196 ABIN6260222 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 17
3.08196 ABIN222888 IP IHC WB Rabbit IgG N-Term Log in to see Polyclonal 4
3.08196 ABIN2787860 WB Rabbit Middle Region Log in to see Polyclonal 0
3.08196 ABIN3044481 ICC IHC (fro) IHC (p) WB Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 91
3.08196 ABIN185269 ELISA WB Goat N-Term Log in to see Polyclonal 1
3.08196 ABIN2792106 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.08196 ABIN2705576 IP IHC WB Rabbit pSer70 Log in to see Polyclonal 0
3.08196 ABIN2705578 IHC WB Rabbit pSer87 Log in to see Polyclonal 0
3.08196 ABIN2705575 IHC WB Rabbit Center Log in to see Polyclonal 0
3.08196 ABIN2705577 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
3.08196 ABIN2705579 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
3.08196 ABIN2705574 IHC WB Rabbit pSer69 Log in to see Polyclonal 0
3.08196 ABIN4283388 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
3.08196 ABIN2862602 IP IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal 0
3.08196 ABIN2862600 IF IP IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal 0
3.08196 ABIN3222748 WB Rabbit Log in to see Polyclonal 0
3.08196 ABIN1532816 IHC ELISA WB Rabbit IgG AA 41-90 Log in to see Polyclonal 1
3.08196 ABIN1531786 ELISA IHC IP WB Rabbit IgG AA 41-90, pSer70 Log in to see Polyclonal 1
3.08196 ABIN1531302 IHC ELISA WB Rabbit IgG AA 61-110, pSer87 Log in to see Polyclonal 1

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 1-211
(251), (79), (64), (45), (40), (29), (28), (27), (20), (17), (15), (15), (15), (10), (10), (10), (10), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1222), (265), (242), (70), (38), (21), (19), (19), (6), (4), (3), (3), (3), (2), (2)
Host Rabbit
(644), (643), (18), (6), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(60), (54), (47), (46), (36), (23), (21), (20), (18), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (10), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Western Blotting (WB)
(973), (446), (445), (442), (379), (357), (206), (155), (148), (125), (81), (42), (33), (15), (13), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 79 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN3042814) Images
Purpose Rabbit IgG polyclonal antibody for Apoptosis regulator Bcl-2(BCL2) detection. Tested with WB, ELISA in Human.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Apoptosis regulator Bcl-2(BCL2) detection. Tested with WB, ELISA in Human.
Gene Name: B-cell CLL/lymphoma 2
Protein Name: Apoptosis regulator Bcl-2
Purification Immunogen affinity purified.
Immunogen E. coli-derived human BCL-2 recombinant protein(Position: M1-D211).
Isotype IgG
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN3042814) Images back to top
Antigen
Alternative Name BCL2 (BCL2 Antibody Abstract)
Background Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14,18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Synonyms: Apoptosis regulator Bcl2 antibody|B cell CLL antibody|B cell lymphoma 2 antibody|B cell lymphoma protein 2 alpha isoform antibody|B cell lymphoma protein 2 antibody|B-cell CLL/lymphoma 2 antibody|B-cell leukemia/lymphoma 2 antibody|Bcl 2 antibody|bcl2a antibody|Leukemia /lymphoma, B-cell, 2 antibody|Oncogene B cell leukemia 2 antibody|Oncogene B-cell leukemia 2 antibody
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN3042814) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Species: Human
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Human

Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN3042814) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-BCL2 antibody (ABIN3042814)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Zong, Li, Lin, Hou, Ma: "Lipoxin A4 pretreatment mitigates skeletal muscle ischemia-reperfusion injury in rats." in: American journal of translational research, Vol. 9, Issue 3, pp. 1139-1150, 2017

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, 2015

Xu, Liu, Zhang, Wang, Wang, Yang, Huo, Sun: "Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy." in: Neural regeneration research, Vol. 7, Issue 17, pp. 1318-24, 2015

Ding, Wang, Zhao, Sun, Zhai: "Protective Effects of Baicalin on A?????-Induced Learning and Memory Deficit, Oxidative Stress, and Apoptosis in Rat." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 623-32, 2015

Yang, Li, Liu, Shi, Zhang: "Inhibitory effect of tetramethylpyrazine preconditioning on overload training-induced myocardial apoptosis in rats." in: Chinese journal of integrative medicine, Vol. 21, Issue 6, pp. 423-30, 2015

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, 2015

Li, Wang, Wang: "Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina." in: Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB), Vol. 31, Issue 4, pp. 352-9, 2015

Zhan, Hu, Yi, An, Huang: "Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4142-8, 2015

Peng, Chen, Chen, Fang, Cui, Zuo, Deng, Chen, Geng, Lai: "Aflatoxin B1 affects apoptosis and expression of Bax, Bcl-2, and Caspase-3 in thymus and bursa of fabricius in broiler chickens." in: Environmental toxicology, 2015

Tang, Zhang, Qi, Chen, Li, Liu, He, Li, Zu: "Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line." in: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1851-1856, 2015

Liu, Su, Zhang, Zhang, Wang, Qian: "Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-?-induced protein 8-like-2 in septic rats." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 386-98, 2015

Huang, Lin, Su, Ye: "Forsythiaside protects against hydrogen peroxide-induced oxidative stress and apoptosis in PC12 cell." in: Neurochemical research, Vol. 40, Issue 1, pp. 27-35, 2015

Yin, Li, Li, Yu, Cai, Zhang, Wang, Zhang, Zhou, Cheng, Gao: "Critical role of prohibitin in endothelial cell apoptosis caused by glycated low-density lipoproteins and protective effects of grape seed procyanidin B2." in: Journal of cardiovascular pharmacology, Vol. 65, Issue 1, pp. 13-21, 2015

Liu, Zheng, Wang, Han, Yuan, Zhao: "Transplantation of olfactory ensheathing cells attenuates acute carbon monoxide poisoning-induced brain damages in rats." in: Neurochemical research, Vol. 40, Issue 1, pp. 70-80, 2015

Wang, Zhang, Chai, Liu, Berk: "The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression." in: BMC psychiatry, Vol. 14, pp. 349, 2015

Wu, Dong, Li: "Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload." in: International journal of molecular medicine, Vol. 35, Issue 4, pp. 893-900, 2015

Sun, Zhang, Guan, Zhou, Sun: "Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice." in: International journal of clinical and experimental medicine, Vol. 8, Issue 1, pp. 358-67, 2015

Han, Yu, Song, Luo, Wu: "Agmatine protects Müller cells from high-concentration glucose-induced cell damage via N-methyl-D-aspartic acid receptor inhibition." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 1098-106, 2015

Gao, Xie, Peng, Han, Jiang, Han, Wang: "Hispidulin inhibits proliferation and enhances chemosensitivity of gallbladder cancer cells by targeting HIF-1?." in: Experimental cell research, Vol. 332, Issue 2, pp. 236-46, 2015

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, 2014

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN3042814) back to top
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 1-211) antibody (ABIN3042814) Western blot analysis of BCL-2 using anti- BCL-2 antibody . Electrophoresis was perf...