anti-Human BCL2 antibody for Western Blotting

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitope
N-Term
248
78
64
60
40
28
28
26
22
21
16
15
15
15
11
11
9
9
9
9
8
7
7
7
7
6
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1257
272
250
70
36
21
21
19
6
4
3
3
3
2
2
Host
Rabbit
702
632
19
6
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
62
57
53
50
43
25
24
24
23
15
14
14
14
14
14
14
13
13
11
11
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1028
474
473
423
410
406
223
188
156
155
81
42
33
15
13
9
6
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN222888
$ 275.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.920577 ABIN388095 IF IHC (p) WB Rabbit Ig Fraction AA 75-110, BH3 Domain Log in to see Polyclonal 19
2.920577 ABIN2787860 WB Rabbit Middle Region Log in to see Polyclonal 0
2.920577 ABIN3044481 ICC IHC (fro) IHC (p) WB Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 91
2.920577 ABIN185269 ELISA WB Goat N-Term Log in to see Polyclonal 1
2.920577 ABIN2792106 IHC WB Rabbit N-Term Log in to see Polyclonal 1
2.920577 ABIN3042814 ELISA WB Rabbit IgG AA 1-211 Log in to see Polyclonal 79
2.920577 ABIN2705576 IP IHC WB Rabbit pSer70 Log in to see Polyclonal 0
2.920577 ABIN2705578 IHC WB Rabbit pSer87 Log in to see Polyclonal 0
2.920577 ABIN2705575 IHC WB Rabbit Center Log in to see Polyclonal 0
2.920577 ABIN2705577 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
2.920577 ABIN2705579 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
2.920577 ABIN2705574 IHC WB Rabbit pSer69 Log in to see Polyclonal 0
2.920577 ABIN4283388 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see 8C8 0
2.920577 ABIN268671 ELISA WB Goat N-Term Log in to see Polyclonal 4
2.920577 ABIN2862602 IP IHC ELISA WB Rabbit IgG pSer70 Log in to see Polyclonal 0
2.920577 ABIN2862600 IF IP IHC ELISA WB Rabbit IgG pThr56 Log in to see Polyclonal 0
2.920577 ABIN3222748 WB Rabbit Log in to see Polyclonal 0
2.920577 ABIN1532816 IHC ELISA WB Rabbit IgG AA 41-90 Log in to see Polyclonal 1
2.920577 ABIN1531786 ELISA IHC IP WB Rabbit IgG AA 41-90, pSer70 Log in to see Polyclonal 1
2.920577 ABIN1531302 IHC ELISA WB Rabbit IgG AA 61-110, pSer87 Log in to see Polyclonal 1

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term
(248), (78), (64), (60), (40), (28), (28), (26), (22), (21), (16), (15), (15), (15), (11), (11), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1257), (272), (250), (70), (36), (21), (21), (19), (6), (4), (3), (3), (3), (2), (2)
Host Rabbit
(702), (632), (19), (6), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(62), (57), (53), (50), (43), (25), (24), (24), (23), (15), (14), (14), (14), (14), (14), (14), (13), (13), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1028), (474), (473), (423), (410), (406), (223), (188), (156), (155), (81), (42), (33), (15), (13), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN222888) Images
Blocking Peptide Blocking peptide for this product available: ABIN412606
Specificity Detects a 25 kDa protein, corresponding to the apparent molecular weight of the ? isoform of Bcl-2 on SDS-PAGE immunoblots.
Purification Affinity purified
Immunogen Synthetic peptide corresponding to the N-terminus of human Bcl-2
Isotype IgG
Plasmids, Primers & others

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 antibody (ABIN222888) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis.
Synonyms: BCL2, BCL 2
Gene ID 596
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 antibody (ABIN222888) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipation (4-8 μg/mL), and Immunohistochemistry (10-20 μg/mL). Detects a 25 kDa protein, corresponding to the apparent molecular weight of the ? isoform of Bcl-2 on SDS-PAGE immunoblots.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 antibody (ABIN222888) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Bcl-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BCL2 antibody (ABIN222888)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Eslami, Sharifi, Rahimi, Rahati: "Protective effect of telmisartan against oxidative damage induced by high glucose in neuronal PC12 cell." in: Neuroscience letters, 2013

Guérin, Cornut-Thibaut, Giscard-Destaing, Pouvreau, Guillemin, Aouacheria: "Subcellular dynamics of the maternal cell death regulator BCL2L10 in human preimplantation embryos." in: Human reproduction (Oxford, England), Vol. 28, Issue 3, pp. 729-39, 2013

Kiran Kumar, Prabhakara Rao, Noble, Weddington, McDowell, Rajanna, Bettaiya: "Lead-induced alteration of apoptotic proteins in different regions of adult rat brain." in: Toxicology letters, Vol. 184, Issue 1, pp. 56-60, 2008

Giuliani, Mioni, Altavilla, Leone, Bazzani, Minutoli, Bitto, Cainazzo, Marini, Zaffe, Botticelli, Pizzala, Savio, Necchi, Schiöth, Bertolini, Squadrito, Guarini: "Both early and delayed treatment with melanocortin 4 receptor-stimulating melanocortins produces neuroprotection in cerebral ischemia." in: Endocrinology, Vol. 147, Issue 3, pp. 1126-35, 2006

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 antibody (ABIN222888) back to top
Supplier Images
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (N-Term) antibody (ABIN222888) Western blot analysis of Bcl-2 expression in HL60 cell lysate.