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|Antigen||Cortactin (CTTN) Antibodies|
|Reactivity||Dog (Canine), Human Alternatives|
|Conjugate||This Cortactin antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|6 references available|
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Product Details anti-Cortactin AntibodyTarget Details Cortactin Application Details Handling References for anti-Cortactin antibody (ABIN967710) Images
|Cross-Reactivity||Human, Dog (Canine)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details CortactinProduct Details anti-Cortactin Antibody Application Details Handling References for anti-Cortactin antibody (ABIN967710) Images back to top
|Alternative Name||Cortactin (CTTN Antibody Abstract)|
|Background||Cortactin is a protein which is represented by two structurally related isoforms of 80 kDa and 85 kDa. Sequence analysis of this protein reveals the presence of a carboxy-terminal SH3 domain, as well as six internal tandem repeats of a 37 amino acid sequence. It is able to bind to F-actin via this N-terminal repeat region, and colocalizes with actin in the cell. Cortactin was originally described as a protein that is tyrosine-phosphorylated in cells transformed by the src oncogene, in which it localizes to rosettes or podosomes. It has since been shown that other stimuli, such as bacterial invasion, treatment with growth factors, and integrin-mediated cell adhesion, are also capable of inducing phosphorylation of cortactin. Some additional evidence indicates that cortactin may actually be a substrate of syk, with src acting as an upstream signaling molecule. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||85/80 kDa|
Application DetailsProduct Details anti-Cortactin Antibody Target Details Cortactin Handling References for anti-Cortactin antibody (ABIN967710) Images back to top
Related Products: ABIN968533, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cortactin Antibody Target Details Cortactin Application Details References for anti-Cortactin antibody (ABIN967710) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-Cortactin antibody (ABIN967710)Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling Images back to top
|Product cited in:||
Cantarelli, Takahashi, Yanagihara, Akeda, Imura, Kodama, Kono, Sato, Iida, Honda: "Cortactin is necessary for F-actin accumulation in pedestal structures induced by enteropathogenic Escherichia coli infection." in: Infection and immunity, Vol. 70, Issue 4, pp. 2206-9, 2002
Lavoie, Champagne, Gingras, Robert: "Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases." in: The Journal of cell biology, Vol. 150, Issue 5, pp. 1037-56, 2000
Nakahara, Mueller, Nomizu, Yamada, Yeh, Chen: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, 1998
Dehio, Prévost, Sansonetti: "Invasion of epithelial cells by Shigella flexneri induces tyrosine phosphorylation of cortactin by a pp60c-src-mediated signalling pathway." in: The EMBO journal, Vol. 14, Issue 11, pp. 2471-82, 1995
Wu, Parsons: "Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1417-26, 1993
Wu, Reynolds, Kanner, Vines, Parsons: "Identification and characterization of a novel cytoskeleton-associated pp60src substrate." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5113-24, 1991
ImagesProduct Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling References for anti-Cortactin antibody (ABIN967710) back to top