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|Antigen||ELK1, Member of ETS Oncogene Family (ELK1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This ELK1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-ELK1 AntibodyTarget Details ELK1 Application Details Handling Images
|Specificity||Specific for ~46k Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.|
Species reactivity (expected):Human, Canine, Mouse, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser383 of human Elk-1.|
|Plasmids, Primers & others|
Target Details ELK1Product Details anti-ELK1 Antibody Application Details Handling Images back to top
|Alternative Name||ELK1 (ELK1 Antibody Abstract)|
|Background||Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).Synonyms: ETS domain-containing protein Elk-1, Elk-1|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-ELK1 Antibody Target Details ELK1 Handling Images back to top
Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|