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|Antigen||Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies|
|Conjugate||This ERK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
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Product Details anti-ERK2 AntibodyTarget Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN400970) Images
|Specificity||This antibody reacts to p42 MAP Kinase (ERK2) protein. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources.|
Species reactivity (expected):Rat.
Species reactivity (tested):Human.
|Immunogen||This whole rabbit antiserum was prepared by repeated immunizations with a GST fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.|
Target Details ERK2Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 antibody (ABIN400970) Images back to top
|Alternative Name||ERK2 (MAPK1 Antibody Abstract)|
|Background||Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the upregulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. In vertebrates the mitogeninduced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 antibody (ABIN400970) Images back to top
ELISA: 1/2000 to 1/10000. Western Blot: 1/1000-1/2000. Immunopreicpitation: 5.0 μg/mg sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 antibody (ABIN400970) Images back to top
|Concentration||85 mg/mL (by Refractometry)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-ERK2 antibody (ABIN400970)Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Asthagiri, Reinhart, Horwitz, Lauffenburger: "The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis." in: Journal of cell science, Vol. 113 Pt 24, pp. 4499-510, 2001
Hoffmann, Baillie, MacKenzie, Yarwood, Houslay: "The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579." in: The EMBO journal, Vol. 18, Issue 4, pp. 893-903, 1999
Garrington, Johnson: "Organization and regulation of mitogen-activated protein kinase signaling pathways." in: Current opinion in cell biology, Vol. 11, Issue 2, pp. 211-8, 1999
Elion: "Routing MAP kinase cascades." in: Science (New York, N.Y.), Vol. 281, Issue 5383, pp. 1625-6, 1998
Pelech, Charest: "MAP kinase-dependent pathways in cell cycle control." in: Progress in cell cycle research, Vol. 1, pp. 33-52, 1998
ImagesProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN400970) back to top