anti-Human ERK2 antibody for Enzyme Immunoassay

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ERK
  • ERK2
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • zERK2
  • MAPK1
  • Xp42
  • erk
  • erk2
  • ert1
  • mapk
  • mapk1b
  • mapk2
  • mpk1
  • prkm1
  • prkm2
  • mapk1
  • mapk1a
  • xp42
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 1
  • mitogen activated protein kinase 1
  • mitogen-activated protein kinase 1 L homeolog
  • mitogen-activated protein kinase 1 S homeolog
  • MAPK1
  • Mapk1
  • mapk1
  • mapk1.L
  • mapk1.S
  • PfMAP1
  • MPK1
  • PVX_084965
  • LOC9328617
  • Tsp_01601
Epitope
C-Term
58
21
16
11
9
8
8
8
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
352
150
119
51
31
19
14
11
10
9
9
7
5
5
2
2
2
2
2
1
1
Host
Rabbit
169
167
16
8
3
Clonality
Polyclonal
Conjugate
This ERK2 antibody is un-conjugated
10
9
8
7
7
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
329
161
96
83
82
60
53
27
23
9
5
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN400970
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Log in to see AT1A4 0
1 ABIN126833 EIA WB Mouse IgG1 N-Term Log in to see 6H3 1
1 ABIN126832 EIA IP WB Mouse IgG1 C-Term Log in to see 6G11 0
1 ABIN5552223 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 0
1 ABIN5552208 EIA IHC (p) WB Rabbit Ig Fraction Middle Region Log in to see Polyclonal 0
1 ABIN400733 EIA IHC (fro) IHC (p) WB Mouse IgG2a Log in to see 4C11C11C4 0
1 ABIN492352 EIA WB Biotin Mouse IgG1 N-Term Log in to see 6H3 0
1 ABIN492351 EIA IP WB Biotin Mouse IgG1 C-Term Log in to see 6G11 0
1 ABIN641241 CA DB EIA IA ISt ELISA WB Rabbit Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope C-Term
(58), (21), (16), (11), (9), (8), (8), (8), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(352), (150), (119), (51), (31), (19), (14), (11), (10), (9), (9), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(169), (167), (16), (8), (3)
Clonality
Polyclonal
Conjugate This ERK2 antibody is un-conjugated
(10), (9), (8), (7), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(329), (161), (96), (83), (82), (60), (53), (27), (23), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN400970) Images
Specificity This antibody reacts to p42 MAP Kinase (ERK2) protein. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources.
Cross-Reactivity (Details) Species reactivity (expected):Rat.
Species reactivity (tested):Human.
Purification Serum
Immunogen This whole rabbit antiserum was prepared by repeated immunizations with a GST fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
Isotype IgG

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 antibody (ABIN400970) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the upregulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. In vertebrates the mitogeninduced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Gene ID 5594
NCBI Accession NP_002736
UniProt P28482
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 antibody (ABIN400970) Images back to top
Application Notes ELISA: 1/2000 to 1/10000. Western Blot: 1/1000-1/2000. Immunopreicpitation: 5.0 μg/mg sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 antibody (ABIN400970) Images back to top
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ERK2 antibody (ABIN400970)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Background publications

Asthagiri, Reinhart, Horwitz, Lauffenburger: "The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis." in: Journal of cell science, Vol. 113 Pt 24, pp. 4499-510, 2001

Hoffmann, Baillie, MacKenzie, Yarwood, Houslay: "The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579." in: The EMBO journal, Vol. 18, Issue 4, pp. 893-903, 1999

Garrington, Johnson: "Organization and regulation of mitogen-activated protein kinase signaling pathways." in: Current opinion in cell biology, Vol. 11, Issue 2, pp. 211-8, 1999

Elion: "Routing MAP kinase cascades." in: Science (New York, N.Y.), Vol. 281, Issue 5383, pp. 1625-6, 1998

Pelech, Charest: "MAP kinase-dependent pathways in cell cycle control." in: Progress in cell cycle research, Vol. 1, pp. 33-52, 1998

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN400970) back to top
Supplier Images
 image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody (ABIN400970) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody