anti-Mouse (Murine) ERK2 antibody for Immunoprecipitation

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ERK
  • ERK2
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • zERK2
  • MAPK1
  • Xp42
  • erk
  • erk2
  • ert1
  • mapk
  • mapk1b
  • mapk2
  • mpk1
  • prkm1
  • prkm2
  • mapk1
  • mapk1a
  • xp42
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 1
  • mitogen activated protein kinase 1
  • mitogen-activated protein kinase 1 L homeolog
  • mitogen-activated protein kinase 1 S homeolog
  • MAPK1
  • Mapk1
  • mapk1
  • mapk1.L
  • mapk1.S
  • PfMAP1
  • MPK1
  • PVX_084965
  • LOC9328617
  • Tsp_01601
Reactivity
Human, Mouse (Murine)
321
132
126
31
29
21
14
10
9
9
9
7
5
4
2
2
2
2
1
1
1
1
Host
Mouse
188
122
18
7
3
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
11
9
7
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
305
150
113
64
59
58
25
24
14
10
7
5
3
3
1
1
1
1
1
1
1
1
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Catalog No. ABIN967397
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.940398 ABIN438932 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 310-360 Log in to see Polyclonal 0
7 ABIN218336 IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
6.5380793 ABIN5950193 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see SZ25-01 0
4 ABIN126832 EIA IP WB Mouse IgG1 C-Term Log in to see 6G11 0
4 ABIN302975 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN1736732 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN342484 IP ELISA WB Rabbit C-Term Log in to see Polyclonal 0
4 ABIN342485 IP ELISA WB Rabbit Tyr182, pThr180 Log in to see Polyclonal 0
4 ABIN2469740 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN492351 EIA IP WB Biotin Mouse IgG1 C-Term Log in to see 6G11 0
1 ABIN596437 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN210913 IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2625132 ELISA IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2742748 ChIP IF IHC IHC (p) IP WB Mouse IgG2a Log in to see 6F8 0
1 ABIN199412 IP ELISA WB Mouse IgG1 Log in to see 0
1 ABIN221449 ICC FACS IF IP ELISA WB Rabbit IgG Tyr182, pThr180 Log in to see 0
1 ABIN220478 FACS IP WB Mouse IgG1 Tyr182, pThr180 Log in to see 0
1 ABIN306318 IP ELISA WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2306383 IP ELISA WB Mouse IgG2a Log in to see 0-T-86 0
1 ABIN2306385 IP WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Reactivity Human, Mouse (Murine)
(321), (132), (126), (31), (29), (21), (14), (10), (9), (9), (9), (7), (5), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(188), (122), (18), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(11), (9), (7), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(305), (150), (113), (64), (59), (58), (25), (24), (14), (10), (7), (5), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN967397) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human ERK2 Synthetic Peptide
Clone G263-7
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 antibody (ABIN967397) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signalling molecules (protein kinases and phosphatases), cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers. The G263-7 recognizes ERK2. It does not cross-react with ERK1. Clone G263-7 was originally characterized in human (A431) and mouse (NIH/3T3) cells.A human ERK2 synthetic peptide was used as immunogen.
Molecular Weight 42 kDa
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 antibody (ABIN967397) Images back to top
Application Notes Applications include western blot analysis (1-2 µg/ml). Store the antibody at 4°C. Other applications not routinely tested include immunoprecipitation (1-2 µg/1x10^6 cells). Human fibroblasts, A431 human epidermal carcinoma cells (ATCC CRL-1555) and NIH/3T3 mouse embryo fibroblasts (ATCC CRL-1658) are suggested as positive controls.
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 antibody (ABIN967397) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-ERK2 antibody (ABIN967397)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Product cited in:

Boulton, Nye, Robbins, Ip, Radziejewska, Morgenbesser, DePinho, Panayotatos, Cobb, Yancopoulos: "ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF." in: Cell, Vol. 65, Issue 4, pp. 663-75, 1991

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN967397) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibody (ABIN967397) Western blot analysis of ERK2. Lysate from Rous Sarcoma Virustransformed mouse 3T3 fi...
 image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibody (ABIN967397) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibody (Image 2)
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibody (ABIN967397) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibody (Image 3)