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|Antigen||Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This ERK2 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-ERK2 AntibodyTarget Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN967397) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human ERK2 Synthetic Peptide|
|Plasmids, Primers & others|
Target Details ERK2Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 antibody (ABIN967397) Images back to top
|Alternative Name||ERK2 (MAPK1 Antibody Abstract)|
|Background||The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signalling molecules (protein kinases and phosphatases), cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers. The G263-7 recognizes ERK2. It does not cross-react with ERK1. Clone G263-7 was originally characterized in human (A431) and mouse (NIH/3T3) cells.A human ERK2 synthetic peptide was used as immunogen.|
|Molecular Weight||42 kDa|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 antibody (ABIN967397) Images back to top
|Application Notes||Applications include western blot analysis (1-2 µg/ml). Store the antibody at 4°C. Other applications not routinely tested include immunoprecipitation (1-2 µg/1x10^6 cells). Human fibroblasts, A431 human epidermal carcinoma cells (ATCC CRL-1555) and NIH/3T3 mouse embryo fibroblasts (ATCC CRL-1658) are suggested as positive controls.|
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 antibody (ABIN967397) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-ERK2 antibody (ABIN967397)Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
|Product cited in:||
Boulton, Nye, Robbins, Ip, Radziejewska, Morgenbesser, DePinho, Panayotatos, Cobb, Yancopoulos: "ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF." in: Cell, Vol. 65, Issue 4, pp. 663-75, 1991
ImagesProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 antibody (ABIN967397) back to top