anti-Chicken HSPB1 antibody for Flow Cytometry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Reactivity
Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
967
373
345
161
132
102
86
67
66
60
47
42
36
29
13
11
7
4
3
2
2
2
1
1
1
1
Host
Mouse
558
427
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
41
39
27
27
22
18
14
12
12
11
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
6
6
6
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
840
429
290
285
260
238
215
211
75
58
42
42
40
18
14
6
5
4
2
2
1
1
1
Supplier
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Catalog No. ABIN3026467
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6164157 FACS IF IHC (f) ISt WB APC Mouse IgG1, kappa Log in to see G3-1 0
7 ABIN6164164 FACS IF IHC (f) ISt WB PerCP Mouse IgG1, kappa Log in to see G3-1 0
7 ABIN6164165 FACS IF IHC (f) ISt WB PE Mouse IgG1, kappa Log in to see G3-1 0
7 ABIN6164195 FACS IF IHC (f) ISt WB APC Mouse IgG1, kappa Log in to see HSPB1-774 0
7 ABIN6164202 FACS IF IHC (f) ISt WB PerCP Mouse IgG1, kappa Log in to see HSPB1-774 0
7 ABIN6164203 FACS IF IHC (f) ISt WB PE Mouse IgG1, kappa Log in to see HSPB1-774 0
7 ABIN6164168 FACS IF IHC (f) ISt WB Mouse IgG1, kappa Log in to see G3-1 0
7 ABIN6164206 FACS IF IHC (f) ISt WB Mouse IgG1, kappa Log in to see HSPB1-774 0
7 ABIN3025018 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see SPM252 0
7 ABIN3025020 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164131 FACS IF IHC (f) ISt WB CF®405S Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164141 FACS IF IHC (f) ISt WB CF®555 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164147 FACS IF IHC (f) ISt WB CF®770 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164151 FACS IF IHC (f) ISt WB CF®680R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164135 FACS IF IHC (f) ISt WB CF®640R Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164139 FACS IF IHC (f) ISt WB CF®647 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164169 FACS IF IHC (f) ISt WB CF®405S Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164145 FACS IF IHC (f) ISt WB CF®568 Mouse IgG1, kappa Log in to see G3-1 0
1 ABIN6164173 FACS IF IHC (f) ISt WB CF®640R Mouse IgG1, kappa Log in to see HSPB1-774 0
1 ABIN6164149 FACS IF IHC (f) ISt WB CF®680 Mouse IgG1, kappa Log in to see G3-1 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(967), (373), (345), (161), (132), (102), (86), (67), (66), (60), (47), (42), (36), (29), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(558), (427), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(41), (39), (27), (27), (22), (18), (14), (12), (12), (11), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(840), (429), (290), (285), (260), (238), (215), (211), (75), (58), (42), (42), (40), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Characteristics This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced "p29" and "24K" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Purification Protein G affinity chromatography
Immunogen Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
Clone G3-1
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background This antibody recognizes a 24-27  kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced ""p29"" and ""24K"" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN3026467) IHC testing of breast carcinoma (20X) stained with HSP27 antibody (G3.1).
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN3026467) IHC testing of FFPE human prostate carcinoma and HSP27 antibody.
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN3026467) IHC testing of human breast carcinoma (10X) stained with HSP27 antibody (G3.1).