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|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Reactivity||Chicken, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling Images
|Specificity||It recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50 % of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.|
|Purification||Protein A purified|
|Immunogen||Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
|Alternative Name||HSP27 (HSPB1 Antibody Abstract)|
|Background||Gene Symbol: HSPB1|
|Molecular Weight||Theoretical MW: 27 kDa|
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
|Application Notes||Western Blot 0.25-0.5 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 μg/mL, Immunohistochemistry-Paraffin 0.5-1.0 μg/mLHuman Chromosome Location: 7q11.23 Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store at 4°C.|