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|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Epitope||AA 1-205, N-Term Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN317528) Images
|Specificity||The antibody recognizes human Hsp27. Other species not tested.|
|Characteristics||Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein|
|Purification||Protein-G affinity chromatography|
|Immunogen||Recombinant human Hsp27 (1-205 aa) purified from E. coli|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN317528) Images back to top
|Alternative Name||HSPB1 / HSP27 (HSPB1 Antibody Abstract)|
|Background||Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27|
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN317528) Images back to top
ELISA. Western blot (1: 1,000 - 1: 2,000). Immunohistochemistry on Parffin embedded tissue (1: 50).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN317528) Images back to top
|Buffer||PBS, pH 7.4, containing 0.09 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-HSPB1 antibody (ABIN317528)Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Borrelli, Bernock, Landry, Spitz, Weber, Hickey, Freeman, Corry: "Stress protection by a fluorescent Hsp27 chimera that is independent of nuclear translocation or multimeric dissociation." in: Cell stress & chaperones, Vol. 7, Issue 3, pp. 281-96, 2002
Butt, Immler, Meyer, Kotlyarov, Laass, Gaestel: "Heat shock protein 27 is a substrate of cGMP-dependent protein kinase in intact human platelets: phosphorylation-induced actin polymerization caused by HSP27 mutants." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7108-13, 2001
Charette, Lavoie, Lambert, Landry: "Inhibition of Daxx-mediated apoptosis by heat shock protein 27." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7602-12, 2000
ImagesProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN317528) back to top