anti-Human HSPB1 antibody for Enzyme Immunoassay

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Epitope
AA 1-205, N-Term
86
73
56
44
22
16
13
13
10
10
10
10
6
6
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
833
278
273
96
85
71
68
66
36
30
29
26
18
11
11
9
6
3
2
2
2
2
1
1
1
Host
Mouse
509
342
2
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
33
23
22
19
13
13
13
11
11
10
10
10
10
10
9
9
9
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
725
337
274
266
232
214
167
158
59
40
17
13
11
8
6
4
2
2
1
1
1
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Catalog No. ABIN317528
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358314 EIA Rabbit Ig Fraction pSer78 Log in to see Polyclonal 5
1 ABIN453119 EIA WB Rabbit Middle Region Log in to see Polyclonal 5
1 ABIN5610927 EIA IHC (p) WB Rabbit Ig Fraction Ser82 Log in to see Polyclonal 0
1 ABIN5610926 EIA IHC (p) WB Rabbit Ig Fraction Ser78 Log in to see Polyclonal 0
1 ABIN5610928 EIA WB Rabbit Ig Fraction Ser83 Log in to see Polyclonal 0
1 ABIN400642 EIA IF IHC (p) IP WB Mouse IgG1 Log in to see n-a 0
1 ABIN265470 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN5611604 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D7 2
1 ABIN5550951 EIA IHC (p) IP WB Mouse IgG2b Log in to see 5D12-A12 0
1 ABIN5550950 EIA IHC (p) IP WB Mouse IgG2b Log in to see 5D12-A12 0
1 ABIN613191 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN613665 EIA RIA Rabbit IgG Log in to see Polyclonal 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 1-205, N-Term
(86), (73), (56), (44), (22), (16), (13), (13), (10), (10), (10), (10), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(833), (278), (273), (96), (85), (71), (68), (66), (36), (30), (29), (26), (18), (11), (11), (9), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(509), (342), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (33), (23), (22), (19), (13), (13), (13), (11), (11), (10), (10), (10), (10), (10), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(725), (337), (274), (266), (232), (214), (167), (158), (59), (40), (17), (13), (11), (8), (6), (4), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN317528) Images
Specificity The antibody recognizes human Hsp27. Other species not tested.
Characteristics Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
Purification Protein-G affinity chromatography
Immunogen Recombinant human Hsp27 (1-205 aa) purified from E. coli
Clone 2A5
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN317528) Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN317528) Images back to top
Application Notes ELISA. Western blot (1: 1,000 - 1: 2,000). Immunohistochemistry on Parffin embedded tissue (1: 50).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN317528) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-HSPB1 antibody (ABIN317528)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Borrelli, Bernock, Landry, Spitz, Weber, Hickey, Freeman, Corry: "Stress protection by a fluorescent Hsp27 chimera that is independent of nuclear translocation or multimeric dissociation." in: Cell stress & chaperones, Vol. 7, Issue 3, pp. 281-96, 2002

Butt, Immler, Meyer, Kotlyarov, Laass, Gaestel: "Heat shock protein 27 is a substrate of cGMP-dependent protein kinase in intact human platelets: phosphorylation-induced actin polymerization caused by HSP27 mutants." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7108-13, 2001

Charette, Lavoie, Lambert, Landry: "Inhibition of Daxx-mediated apoptosis by heat shock protein 27." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7602-12, 2000

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN317528) back to top
Supplier Images
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody (ABIN317528) anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody (ABIN317528) anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody (ABIN317528) anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody (ABIN317528) anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 1-205), (N-Term) antibody (Image 4)