anti-Mouse (Murine) HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • 27kDa
  • Hsp27
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • heat shock protein family B (small) member 1
  • heat shock protein 1
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock protein family B (small) member 1 L homeolog
  • HSPB1
  • Hspb1
  • hspb1
  • hspb1.L
Epitope
AA 100-150
83
68
58
26
22
17
17
15
15
13
12
9
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
968
373
345
162
133
102
86
67
67
61
47
42
36
29
13
11
7
4
3
2
2
2
1
1
1
1
Host
Rabbit
557
429
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
41
39
27
27
22
18
14
12
12
11
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
6
6
6
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
841
429
290
286
261
239
215
211
75
59
42
42
39
18
14
6
5
4
2
2
1
1
1
Options
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Catalog No. ABIN672441
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.576218 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
12.576219 ABIN1689956 IHC (p) WB Rabbit Log in to see Polyclonal 0
11.119598 ABIN498746 IHC (p) WB Rabbit pSer82 Log in to see Polyclonal 0
11.119598 ABIN498463 IHC (p) WB Rabbit Log in to see Polyclonal 0
11.119598 ABIN5611604 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN3026467 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see G3-1 0
10 ABIN3031144 ICC IHC (fro) IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal 0
8.119598 ABIN498848 IHC (p) Rabbit pSer78, pSer82 Log in to see Polyclonal 0
8.119598 ABIN1107613 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
8.119598 ABIN498937 IHC (p) Rabbit Log in to see Polyclonal 0
7 ABIN197344 IHC (p) WB Rabbit Ser15 Log in to see Polyclonal 4
7 ABIN3025018 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see SPM252 0
7 ABIN3025020 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774 0
7 ABIN3024930 IF IHC (p) WB Mouse IgG1, kappa Log in to see SRPR27 0
5.5 ABIN3043648 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see IAP-9 1
4 ABIN2878840 ELISA IHC IHC (p) WB Rabbit IgG AA 48-97 Log in to see Polyclonal 0
4 ABIN5956880 ELISA IF IHC (p) WB Rabbit IgG Ser15 Log in to see Polyclonal 0
4 ABIN5956886 ELISA IHC (p) WB Rabbit IgG pSer82 Log in to see Polyclonal 0
4 ABIN1493063 IHC IHC (p) Rabbit C-Term Log in to see Polyclonal 0
4 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 100-150
(83), (68), (58), (26), (22), (17), (17), (15), (15), (13), (12), (9), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(968), (373), (345), (162), (133), (102), (86), (67), (67), (61), (47), (42), (36), (29), (13), (11), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(557), (429), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(41), (39), (27), (27), (22), (18), (14), (12), (12), (11), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(841), (429), (290), (286), (261), (239), (215), (211), (75), (59), (42), (42), (39), (18), (14), (6), (5), (4), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN672441) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human HSP27
Isotype IgG
Plasmids, Primers & others

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN672441) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background

Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.

Subcellular location: Cytoplasm, Nucleus

Synonyms: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.7667, HEL-S-12, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27

Gene ID 3315
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN672441) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN672441) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-HSPB1 antibody (ABIN672441)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Gao, Liu, Chen, Lv, Wu, Mi, Wang: "Comparative study of Hsp27, GSK3?, Wnt1 and PRDX3 in Hirschsprung's disease." in: International journal of experimental pathology, Vol. 95, Issue 3, pp. 229-37, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human). Further details: Sample: Colon

Wang, Yang, Liu, Zhou, Wu, Qiao, Li, Wang: "Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets." in: The Journal of nutrition, Vol. 142, Issue 1, pp. 7-13, 2011

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN672441) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/H...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with (ABIN672441) Rabbit Ant...
SDS-PAGE (SDS) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27/HspB1/HSP25 Poly...
Immunofluorescence (IF) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27/HspB1/HSP25 Po...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/H...
 image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 100-150) antibody (ABIN672441) A431 cells probed with HSP27 Polyclonal Antibody, unconjugated at 1:100 dilution for...