anti-Human MAP2K1 antibody for Enzyme Immunoassay

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1 L homeolog
  • MAP kinase/ ERK kinase 1
  • Map2k1
  • MAP2K1
  • map2k1.L
  • map2k1
  • MEK1
Epitope
pSer217, pSer221
39
39
38
35
35
32
32
32
27
26
19
18
16
15
15
15
11
11
10
10
8
7
6
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
712
463
411
105
81
44
30
24
21
19
10
8
5
5
4
4
4
1
1
Host
Rabbit
584
128
5
3
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
21
20
19
12
10
9
9
8
6
6
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
572
284
207
132
131
79
30
24
24
10
10
8
6
6
6
2
1
1
1
1
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Catalog No. ABIN358157
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358538 EIA Rabbit Ig Fraction pSer222 Log in to see Polyclonal 4
1 ABIN126836 EIA WB Mouse IgG1 pSer218, pSer222, pSer226 Log in to see 7-00E-010 0
1 ABIN126835 EIA WB Mouse IgG1 Log in to see 10B1 0
1 ABIN126837 EIA WB Mouse IgG2a Log in to see 9G3 0
1 ABIN5552287 EIA WB Rabbit Ig Fraction Ser216 Log in to see Polyclonal 0
1 ABIN5611760 EIA IHC (p) WB Rabbit IgG AA 189-238, Internal Region Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pSer217, pSer221
(39), (39), (38), (35), (35), (32), (32), (32), (27), (26), (19), (18), (16), (15), (15), (15), (11), (11), (10), (10), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(712), (463), (411), (105), (81), (44), (30), (24), (21), (19), (10), (8), (5), (5), (4), (4), (4), (1), (1)
Host Rabbit
(584), (128), (5), (3), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(21), (20), (19), (12), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(572), (284), (207), (132), (131), (79), (30), (24), (24), (10), (10), (8), (6), (6), (6), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN358157) Images
Specificity This antibody detects MAP2K1/MEK1 pSer217/221. Predicted to cross react with Mouse and Rabbit (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S217/221 of human MEK1.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 antibody (ABIN358157) Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Molecular Weight 44424 Da
Gene ID 5604, 9606
UniProt Q02750
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 antibody (ABIN358157) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 antibody (ABIN358157) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP2K1 antibody (ABIN358157)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Background publications

Ohren, Chen, Pavlovsky, Whitehead, Zhang, Kuffa, Yan, McConnell, Spessard, Banotai, Mueller, Delaney, Omer, Sebolt-Leopold, Dudley, Leung, Flamme, Warmus, Kaufman, Barrett, Tecle, Hasemann: "Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition." in: Nature structural & molecular biology, Vol. 11, Issue 12, pp. 1192-7, 2004

Naegele, Morley: "Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic stress." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46023-34, 2004

Ussar, Voss: "MEK1 and MEK2, different regulators of the G1/S transition." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43861-9, 2004

Spence, Dhillon, James, Winder: "Dystroglycan, a scaffold for the ERK-MAP kinase cascade." in: EMBO reports, Vol. 5, Issue 5, pp. 484-9, 2004

Beausoleil, Jedrychowski, Schwartz, Elias, Villén, Li, Cohn, Cantley, Gygi: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN358157) back to top
Supplier Images
 image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer217), (pSer221) antibody (ABIN358157) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer217), (pSer221) antibody
 image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer217), (pSer221) antibody (ABIN358157) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer217), (pSer221) antibody (Image 2)