anti-Rat (Rattus) MAP2K1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1 L homeolog
  • MAP kinase/ ERK kinase 1
  • Map2k1
  • MAP2K1
  • map2k1.L
  • map2k1
  • MEK1
Epitope
AA 2-35
40
40
38
37
35
34
31
31
26
25
19
18
17
15
15
14
11
11
10
10
8
7
6
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
711
463
412
107
81
46
30
25
21
20
10
8
5
5
4
4
4
1
1
Host
Rabbit
587
126
5
3
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
20
20
19
12
9
9
9
8
6
6
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
575
290
206
138
127
77
31
24
20
10
10
8
7
6
4
2
1
1
1
1
Options
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Available images

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482)
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482)
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482)
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482)
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482)
Catalog No. ABIN686482
$ 306.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.982011 ABIN4333486 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
12.982011 ABIN615073 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
12.982011 ABIN498700 IF IHC (p) WB Rabbit pThr292 Log in to see Polyclonal 0
12.982011 ABIN498720 IHC (p) WB Rabbit pSer218, pSer222 Log in to see Polyclonal 0
12.982011 ABIN498721 IHC (p) WB Rabbit pSer222, pSer226 Log in to see Polyclonal 0
12.982011 ABIN498855 IHC (p) Rabbit pSer222 Log in to see Polyclonal 0
12.982011 ABIN498854 IHC (p) Rabbit pSer218 Log in to see Polyclonal 0
12.982011 ABIN461964 IF IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
12.982011 ABIN498654 IHC (p) WB Rabbit Log in to see Polyclonal 0
12.982011 ABIN498942 IHC (p) Rabbit Log in to see Polyclonal 0
12.982011 ABIN498600 IHC (p) WB Rabbit Log in to see Polyclonal 0
12.982011 ABIN498943 IHC (p) Rabbit Log in to see Polyclonal 0
12.982011 ABIN743798 IF (p) IHC (p) WB Rabbit IgG pThr286 Log in to see Polyclonal 0
12.982011 ABIN4333480 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
12.982011 ABIN4333482 ICC IF IHC IHC (p) WB Rabbit pThr292 Log in to see Polyclonal 0
12.982011 ABIN4333481 IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN1499381 IF IHC IHC (p) WB Mouse IgG1 Log in to see 3H3 0
1 ABIN1499392 IF IHC IHC (p) WB Mouse IgG2b Log in to see 2A1 0
1 ABIN1499386 IF IHC IHC (p) WB Mouse IgG1 Log in to see 6C6 0
1 ABIN1499376 IF IHC IHC (p) WB Mouse IgG2a Log in to see 2B2 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope AA 2-35
(40), (40), (38), (37), (35), (34), (31), (31), (26), (25), (19), (18), (17), (15), (15), (14), (11), (11), (10), (10), (8), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(711), (463), (412), (107), (81), (46), (30), (25), (21), (20), (10), (8), (5), (5), (4), (4), (4), (1), (1)
Host Rabbit
(587), (126), (5), (3), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(20), (20), (19), (12), (9), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(575), (290), (206), (138), (127), (77), (31), (24), (20), (10), (10), (8), (7), (6), (4), (2), (1), (1), (1), (1)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human MEK1
Isotype IgG
Plasmids, Primers & others

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Mek1 (MAP2K1 Antibody Abstract)
Background

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Synonyms: CFC3, MEK1, MKK1, MAPKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1
Gene ID 5604
UniProt Q02750
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Images provided the Independent Validation Program (badge number 029646)Formalin-fixe...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by bo...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Image provided by the Independent Validation Program (badge number 29760). Lane 1: He...
'Independent Validation' Badge
Lot Number 90915
Method validated Immunohistochemistry
Positive Control Human placenta
Negative Control Human breast adipocytes
Notes Signal detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antigen: Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
  • Catalog number: ABIN686482
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 90915
Isotype Control Antibody
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: D07640BA
Controls
  • Positive control: Human placenta tissue stained with antibody
  • Negative control: Human breast adipocytes tissue stained with antibody
  • Isotype control: Human placenta tissue stained with isotype control
  • Secondary only control: Human placenta tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 1: Human placenta tissue stained with anti-MAP2K1 (brown) and counterstained w...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 2: Human breast adipose tissue stained with anti-MAP2K1 (brown) and countersta...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 3: Human placenta tissue stained with isotype control antibody (brown) and cou...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 4: Human placenta tissue stained with secondary antibody only (brown) and coun...