anti-Rat (Rattus) MAP2K1 antibody for Simple Western

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1 L homeolog
  • MAP kinase/ ERK kinase 1
  • Map2k1
  • MAP2K1
  • map2k1.L
  • map2k1
  • MEK1
Epitope
pThr386
39
39
38
35
35
33
32
32
27
26
19
18
16
15
15
15
11
11
10
10
8
7
6
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
712
463
410
105
81
44
30
24
21
19
10
8
5
5
4
4
4
1
1
Host
Rabbit
584
128
5
3
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
21
20
19
12
10
9
9
8
6
6
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Simple Western (SimWes), Western Blotting (WB)
572
284
207
133
131
79
30
24
24
10
10
8
7
6
6
2
1
1
1
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Catalog No. ABIN152969
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General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pThr386
(39), (39), (38), (35), (35), (33), (32), (32), (27), (26), (19), (18), (16), (15), (15), (15), (11), (11), (10), (10), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(712), (463), (410), (105), (81), (44), (30), (24), (21), (19), (10), (8), (5), (5), (4), (4), (4), (1), (1)
Host Rabbit
(584), (128), (5), (3), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(21), (20), (19), (12), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Simple Western (SimWes), Western Blotting (WB)
(572), (284), (207), (133), (131), (79), (30), (24), (24), (10), (10), (8), (7), (6), (6), (2), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN152969) Images
Specificity Specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Purification Immunogen affinity purified
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH
Plasmids, Primers & others

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 antibody (ABIN152969) Images back to top
Antigen
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background Gene Symbol: MAP2K1
Gene ID 5604
UniProt Q02750
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 antibody (ABIN152969) Images back to top
Application Notes Western Blot 1:1000, Simple Western 1:50NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. The investigator should determine the optimal dilution for a specific application. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-All.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 antibody (ABIN152969) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 0.15M NaCl, 0.1 mg/mL BSA and 50 % Glycerol
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-MAP2K1 antibody (ABIN152969)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Product cited in:

Chong, Vikis, Guan: "Mechanisms of regulating the Raf kinase family." in: Cellular signalling, Vol. 15, Issue 5, pp. 463-9, 2003

Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002

Mansour, Resing, Candi, Hermann, Gloor, Herskind, Wartmann, Davis, Ahn: "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 304-14, 1995

Ahn: "The MAP kinase cascade. Discovery of a new signal transduction pathway." in: Molecular and cellular biochemistry, Vol. 127-128, pp. 201-9, 1994

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992

Kyriakis, Brautigan, Ingebritsen, Avruch: "pp54 microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10043-6, 1991

Seger, Ahn, Boulton, Yancopoulos, Panayotatos, Radziejewska, Ericsson, Bratlien, Cobb, Krebs: "Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 14, pp. 6142-6, 1991

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN152969) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody (ABIN152969) Western Blots. Western Blot of recombinant WT and mutant MEK 1 immunolabeled with th...