anti-Human MAP2K2 antibody for Western Blotting

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • CFC4
  • MAPKK2
  • MEK2
  • MKK2
  • PRKMK2
  • AA589381
  • MK2
  • Prkmk2
  • ATMKK2
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MK1
  • zgc:172250
  • mitogen-activated protein kinase kinase 2
  • mitogen activated protein kinase kinase 2
  • MAP kinase kinase 2
  • mitogen-activated protein kinase kinase 2b
  • MAP2K2
  • Map2k2
  • MKK2
  • map2k2b
Epitope
AA 1-50
38
27
19
16
16
14
14
12
11
10
10
7
5
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
315
176
138
13
12
4
3
2
1
1
Host
Rabbit
248
58
13
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
16
15
15
7
7
7
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
271
120
119
49
47
39
26
19
15
13
12
11
4
1
Options
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Available images

Catalog No. ABIN726500
$ 306.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0806582 ABIN1881526 IHC (p) WB Rabbit AA 200-229, Ser222 Log in to see Polyclonal 5
3.0806582 ABIN1882178 IF WB Rabbit AA 1-30, N-Term Log in to see Polyclonal 5
3.0806582 ABIN2783572 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.0806582 ABIN2783573 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.0806582 ABIN2869479 ELISA FACS ICC WB Mouse IgG1 Log in to see 7F5 3
3.0806582 ABIN1531905 ELISA IHC IP WB Rabbit IgG AA 261-310 Log in to see Polyclonal 2
3.0806582 ABIN2704989 IP IHC WB Rabbit C-Term, pSer394 Log in to see Polyclonal 0
3.0806582 ABIN392485 IF IHC (p) WB Rabbit Ig Fraction AA 262-292, Center Log in to see Polyclonal 0
3.0806582 ABIN2855313 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
3.0806582 ABIN2855318 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
3.0806582 ABIN3185526 ELISA IHC IP WB Rabbit IgG Thr38 Log in to see Polyclonal 0
3.0806582 ABIN3182068 ELISA IHC IP WB Rabbit IgG pThr394 Log in to see Polyclonal 0
3.0806582 ABIN5950421 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-100 Log in to see SD207-08 0
3.0806582 ABIN5950422 ICC IF IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see SR13-07 0
3.0806582 ABIN126838 EIA WB Mouse IgG1 Log in to see 8-00E-008 0
3.0806582 ABIN519254 IF IP WB Rabbit AA 1-400, full length Log in to see Polyclonal 0
3.0806582 ABIN5596866 ELISA WB Biotin Mouse IgG C-Term Log in to see 12A6-G1-G11 0
3.0806582 ABIN5596869 ELISA WB Biotin Mouse IgG N-Term Log in to see 19G10-F1-E2 0
3.0806582 ABIN1870344 IF IHC WB Rabbit IgG pThr394 Log in to see Polyclonal 0
3.0806582 ABIN1844273 ELISA FACS IF/ICC WB Mouse IgG1 Log in to see 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope AA 1-50
(38), (27), (19), (16), (16), (14), (14), (12), (11), (10), (10), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(315), (176), (138), (13), (12), (4), (3), (2), (1), (1)
Host Rabbit
(248), (58), (13), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(16), (15), (15), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(271), (120), (119), (49), (47), (39), (26), (19), (15), (13), (12), (11), (4), (1)
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human MAPKK2/MEK2
Isotype IgG
Plasmids, Primers & others

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEK2 (MAP2K2 Antibody Abstract)
Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

Subcellular location: Intracellular

Synonyms: CFC4, MEK2, MKK2, MAPKK2, PRKMK2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2

Gene ID 5605
UniProt P36507
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPK...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250....
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) Images provided by the Independent Validation Program, badge number 029748. Lane one:...
'Independent Validation' Badge
Lot Number 110511
Method validated Western Blotting
Positive Control Hela cell extract
Negative Control c6/36 Mosquito cell extract (non-reactive species)
Notes A single positive band at the correct molecular weight was detected in positive control HeLa cell extract. Several bands were observed in the non-reactive species negative control at approximately the same molecular weight as MAP2K2. These bands may constitute cross-reactivity of the target antigen, or they may be non-specific bands.
Primary Antibody
  • Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (1:150 dilution)
  • Catalog number: ABIN726500
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  • Supplier catalog number: Log in to see
  • Lot number: 110511
Secondary Antibody
  • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
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  • Catalog number: Log in to see
  • Lot number: L170-6515
Controls
  • Positive control: HeLa cell extract
  • Negative control: c6/36 Mosquito cell extract
Protocol
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 46 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution (anti-MAP2K2; 1:150) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 h at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 min each.
  • 11. The membrane was rinsed in TBS twice for 30 s each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • 15. The membrane was washed in TBST 2 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - No challenges noted.
Validation Images
Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) p...