|Antigen||Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies|
|Epitope||C-Term, pSer732 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Horse (Equine), Human, Mouse (Murine), Opossum (Didelphimorphia), Rat (Rattus) Alternatives|
|Conjugate||This RPS6KA1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
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Product Details anti-RPS6KA1 AntibodyTarget Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 antibody (ABIN401473) Images
|Specificity||This antibody is specific for p90 RSK1 protein phosphorylated at S732.|
Species reactivity (expected):Rat, Mouse, Horse, Bovine, Canine, Opossum, Chicken.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein|
Target Details RPS6KA1Product Details anti-RPS6KA1 Antibody Application Details Handling References for anti-RPS6KA1 antibody (ABIN401473) Images back to top
|Alternative Name||RPS6KA1 (RPS6KA1 Antibody Abstract)|
|Background||Ribosomal S6 Kinase 1 (RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK.Synonyms: 90 kDa ribosomal protein S6 kinase 1, MAPKAPK1A, RSK-1, RSK1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase alpha-1, p90RSK1|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling References for anti-RPS6KA1 antibody (ABIN401473) Images back to top
ELISA: 1/100,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details References for anti-RPS6KA1 antibody (ABIN401473) Images back to top
|Concentration||0.64 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-RPS6KA1 antibody (ABIN401473)Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling Images back to top
Chaturvedi, Poppleton, Stringfield, Barbier, Patel: "Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4586-600, 2006
ImagesProduct Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 antibody (ABIN401473) back to top