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General |
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Antigen | Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3) Antibodies |
Epitope | AA 604-617 Alternatives |
Reactivity | Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This RPS6KA3 antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-RPS6KA3 AntibodyTarget Details RPS6KA3 Application Details Handling Images |
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Brand | IHC-plus™ |
Specificity | Detects an ~90 kD protein, corresponding to the apparent molecular mass of RSK2 on SDS-PAGE immunoblots, in samples from mouse and rat origins. |
Predicted Reactivity | Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%). |
Purification | Protein A purified |
Immunogen |
A 14 residue synthetic peptide (C)NRNQSPVLEPVGRS based on the mouse RSK2 S6 kinase (amino acids 604-617) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%). Type of Immunogen: Synthetic peptide - KLH conjugated |
Plasmids, Primers & others | |
Target Details RPS6KA3Product Details anti-RPS6KA3 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | RPS6KA3 / RSK2 (RPS6KA3 Antibody Abstract) |
Background |
Name/Gene ID: RPS6KA3 Subfamily: RSK Family: Protein Kinase Synonyms: RPS6KA3, Coffin-Lowry syndrome, HU-2, ISPK1, MAPKAP kinase 1B, MAPKAPK-1b, MRX19, Ribosomal S6 kinase 2, RSK-2, p90-RSK 3, p90RSK3, RSK, RSK2, S6K-alpha-3, S6K-alpha3, CLS, ISPK-1, MAPKAPK1B, p90-RSK2, Pp90RSK2 |
Gene ID | 6197 |
UniProt | P51812 |
Pathways | MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades |
Application DetailsProduct Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Handling Images back to top |
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Application Notes |
Approved: IHC, IHC-P (20 μg/mL), IP, WB (1 μg/mL) Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL. |
Comment |
Target Species of Antibody: Mouse |
Restrictions | For Research Use only |
HandlingProduct Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Images back to top |
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Format | Liquid |
Concentration | Lot specific |
Buffer | PBS, pH 7.2, 50 % glycerol, 0.09 % azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap. |
Storage | 4 °C,-20 °C |
Storage Comment | Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap. |
ImagesProduct Details anti-RPS6KA3 Antibody Target Details RPS6KA3 Application Details Handling back to top |
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Supplier Images |