anti-Plant TUBB antibody for Immunofluorescence
Recommended TUBB Antibody (supplied by: Log in to see )
- Antigen
-
Tubulin, beta (TUBB) Antibodies
show synonyms for this antigen- M40
- OK/SW-cl.56
- TUBB1
- TUBB5
- XLOT
- m40
- ok/sw-cl.56
- tubb1
- tubb5
- TUBB2A
- TUBB2B
- TUBB
- 1t
- B1t
- BETA 56D
- CG9277
- DTB2
- Dmbeta1
- Dmel\\CG9277
- T
- Tub
- Tubulin
- beta
- beta-Tub
- beta-Tub56D
- beta-tub
- beta-tubulin56D
- beta1
- beta1-Tubulin
- beta1-tub
- beta1Tub
- beta1t
- beta1tub
- beta56D
- betaTub
- betaTub1
- beta[[1]] tubulin
- beta[[1]]-tubulin
- betatub(56D)
- 143391_i_at
- 3t
- B3t
- BETA 60D
- CG3401
- D.m.BETA-60D
- DTB3
- Dmbeta3
- Dmel\\CG3401
- Tub60D
- beta-Tub60D
- beta-Tub6D
- beta3
- beta3 TU
- beta3-Tub
- beta3-tubulin
- beta3TUB
- beta3Tub
- beta3t
- beta60C
- betaTub3
- betaTub60C
- beta[[3]] tubulin
- beta[[3]]-Tub
- beta[[3]]-tubulin
- betatub60D
- p50
- p50/tubulin
- p53
- p53/tubulin
- 2t
- B2t
- BETA 85D
- BETA2
- CG9359
- D.m.BETA-85D
- DTB4
- Dmbeta2
- Dmel\\CG9359
- beta(2)Tu
- beta(2)Tub
- beta-Tub85D
- beta-tub85D
- beta2
- beta2-tub
- beta2t
- beta2tub
- beta85D
- betaTub2
- beta[[2]]-tubulin
- ms(3)KK[D]
- 4t
- B4t
- BETA 98B
- CG4869
- DTB1
- Dmbeta4
- Dmel\\CG4869
- beta-Tub97EF
- beta4
- beta4t
- beta97F
- betaTub4
- betaTub98BC
- betaTub98C
- Tub1
- bmtub1
- Tub3
- bmtub3
- Tub2
- bmtub2
- Tub4
- bmtub4
- LOC100101153
- tubulin beta class I
- putative cobalt-zinc-cadmium outer membrane resistance protein
- Tubulin beta chain
- tubulin beta-2A chain
- beta-Tubulin at 56D
- beta-Tubulin at 60D
- beta-Tubulin at 85D
- beta-Tubulin at 97EF
- beta-tubulin
- beta tubulin
- tubulin beta class I L homeolog
- tubulin, beta 5 class I
- TUBB
- czcC
- tbb-6
- tubb
- LOC462399
- betaTub56D
- betaTub60D
- betaTub85D
- betaTub97EF
- Tub1
- Tub3
- Tub2
- Tub4
- TVAG_525430
- TVAG_062880
- TVAG_456920
- LOC100101153
- Tb927.1.2330
- Tb927.1.2350
- Tb927.1.2370
- Tb927.1.2390
- tubb.L
- Tubb
- LOC693049
- LOC443015
- Tubb5
- Reactivity
-
Human, Mouse (Murine), Plant, Pig (Porcine)
Alternatives - Host
-
Mouse
Alternatives - Clonality
- Conjugate
-
This TUBB antibody is un-conjugated
Alternatives - Application
-
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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Catalog No. ABIN238519
$ 286.00
Plus shipping costs $45.00
Plus shipping costs $45.00
General |
|
|---|---|
| Antigen | Tubulin, beta (TUBB) Antibodies |
| Reactivity | Human, Mouse (Murine), Plant, Pig (Porcine) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Conjugate | This TUBB antibody is un-conjugated Alternatives |
| Application |
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
Alternatives
|
|
3 references available |
| Supplier | Log in to see |
Product Details anti-TUBB AntibodyTarget Details TUBB Application Details Handling References for anti-TUBB antibody (ABIN238519) Images |
|
| Specificity | The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species. |
| Characteristics | Synonyms: Tubulin beta chain, Tubulin beta-5 chain |
| Purification | Precipitation Methods and Size-Exclusion Chromatography. |
| Immunogen | beta-tubulin from porcine brain. |
| Clone | TU-13 |
| Isotype | IgM |
| Plasmids, Primers & others | |
Target Details TUBBProduct Details anti-TUBB Antibody Application Details Handling References for anti-TUBB antibody (ABIN238519) Images back to top |
|
| Antigen | |
| Alternative Name | TUBB / TUBB5 (TUBB Antibody Abstract) |
| Background | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.Synonyms: Tubulin beta chain, Tubulin beta-5 chain |
| Gene ID | 203068 |
| UniProt | P07437 |
| Pathways | Microtubule Dynamics, M Phase |
Application DetailsProduct Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB antibody (ABIN238519) Images back to top |
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| Application Notes | Western Blotting (Reducing Conditions): 1 μg/mL, Sonication of MCF7 cells isrecommended. Positive Control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa |
| Restrictions | For Research Use only |
HandlingProduct Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB antibody (ABIN238519) Images back to top |
|
| Concentration | 1.0 mg/mL |
| Buffer | PBS, pH 7.4 with 15 mM Sodium Azide as preservative. |
| Preservative | Sodium azide |
| Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Storage | 4 °C/-20 °C |
| Storage Comment |
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch |
| Expiry Date | 12 months |
References for anti-TUBB antibody (ABIN238519)Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top |
|
| Background publications |
Linhartová, Dráberová, Viklický, Dráber: "Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells." in: FEBS letters, Vol. 320, Issue 1, pp. 79-82, 1993 Dráber, Vater, Böhm, Kuklova, Unger: "Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies." in: FEBS letters, Vol. 262, Issue 2, pp. 209-11, 1990 Dráber, Lagunowich, Dráberová, Viklický, Damjanov: "Heterogeneity of tubulin epitopes in mouse fetal tissues." in: Histochemistry, Vol. 89, Issue 5, pp. 485-92, 1988 |
