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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN238519 | EIA IHC (fro) IF WB | Mouse | IgM | Log in to see | TU-13 | 3 |
General |
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Antigen | Tubulin, beta (TUBB) Antibodies |
Epitope | AA 81-95 Alternatives |
Reactivity | Human, Mouse (Murine), Plant, Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This TUBB antibody is un-conjugated Alternatives |
Application |
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
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2 references available |
Supplier | Log in to see |
Product Details anti-TUBB AntibodyTarget Details TUBB Application Details Handling References for anti-TUBB antibody (ABIN267968) Images |
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Specificity | The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species. |
Purification | Ammonium sulfate precipitation |
Immunogen | Beta-subunits of porcine brain tubulin. |
Clone | TU-06 |
Isotype | IgM |
Target Details TUBBProduct Details anti-TUBB Antibody Application Details Handling References for anti-TUBB antibody (ABIN267968) Images back to top |
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Antigen | |
Alternative Name | beta Tubulin (TUBB Antibody Abstract) |
Background | Gene Symbol: TUBB |
Gene ID | 203068 |
UniProt | Q767L7 |
Pathways | Microtubule Dynamics, M Phase |
Application DetailsProduct Details anti-TUBB Antibody Target Details TUBB Handling References for anti-TUBB antibody (ABIN267968) Images back to top |
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Application Notes | Western Blot 1 μg/mL, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 2 μg/mL, Immunohistochemistry-Paraffin 5 μg/mLWestern Blot: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunocytochemistry - Staining technique: fixed and permeabilized cells |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-TUBB Antibody Target Details TUBB Application Details References for anti-TUBB antibody (ABIN267968) Images back to top |
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Format | Liquid |
Concentration | 1.0 mg/mL |
Buffer |
TBS (approx. pH 8.0) Buffer contains: 15 mM Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | 4 °C |
Storage Comment | Store at 4°C. Do not freeze. |
References for anti-TUBB antibody (ABIN267968)Product Details anti-TUBB Antibody Target Details TUBB Application Details Handling Images back to top |
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Product cited in: |
Gzyl, Chmielowska-Bąk, Przymusiński, Gwóźdź: "Cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (Glycine max L.)." in: Frontiers in plant science, Vol. 6, pp. 937, 2015 (Sample species: Plant). Further details: Western Blotting Lee, Mullen Grey, Riddick: "Loss of hepatic aryl hydrocarbon receptor protein in adrenalectomized rats does not involve altered levels of the receptor's cytoplasmic chaperones." in: Canadian journal of physiology and pharmacology, Vol. 91, Issue 12, pp. 1154-7, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting |
ImagesProduct Details anti-TUBB Antibody Target Details TUBB Application Details Handling References for anti-TUBB antibody (ABIN267968) back to top |
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Supplier Images |