anti-Horse (Equine) Chromogranin A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Epitope
C-Term
30
17
15
15
12
8
8
6
5
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
1006
310
272
198
154
125
11
11
6
5
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
684
322
23
7
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
41
36
32
32
30
17
17
16
16
16
16
16
16
16
16
16
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
13
8
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
676
622
494
428
290
218
214
212
168
102
16
13
13
8
7
3
2
1
1
1
Supplier
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Catalog No. ABIN214090
$ 727.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2472988 IHC (p) ELISA Sheep IgG Log in to see Polyclonal 0

General

Antigen Chromogranin A (CHGA) Antibodies
Epitope C-Term
(30), (17), (15), (15), (12), (8), (8), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(1006), (310), (272), (198), (154), (125), (11), (11), (6), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(684), (322), (23), (7), (1), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(41), (36), (32), (32), (30), (17), (17), (16), (16), (16), (16), (16), (16), (16), (16), (16), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (13), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(676), (622), (494), (428), (290), (218), (214), (212), (168), (102), (16), (13), (13), (8), (7), (3), (2), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Brand IHC-plus™
Specificity Human CHGA / Chromogranin A
Predicted Reactivity Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide from C-Terminus of human CHGA (P10645, NP_001266). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Lizard (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details

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Antigen
Alternative Name CHGA / Chromogranin A (CHGA Antibody Abstract)
Background Name/Gene ID: CHGA

Synonyms: CHGA, Chromogranin A, Chromogranin-A, Pancreastatin, Pituitary secretory protein I, Vasostatin I, SP-I, Vasostatin II
Gene ID 1113
NCBI Accession NP_001266
UniProt P10645
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

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Format Lyophilized
Reconstitution After adding water, will consist of PBS buffer with 2 % sucrose
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Chromogranin A (CHGA) (C-Term) antibody (ABIN214090) Human Adrenal (formalin-fixed, paraffin-embedded) stained with CHGA antibody ABIN2140...