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|Antigen||Chromogranin A (CHGA) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||Human CHGA / Chromogranin A|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%).|
Synthetic peptide from C-Terminus of human CHGA (P10645, NP_001266). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Lizard (92%), Platypus (85%).
Type of Immunogen: Synthetic peptide
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|Alternative Name||CHGA / Chromogranin A (CHGA Antibody Abstract)|
Name/Gene ID: CHGA
Synonyms: CHGA, Chromogranin A, Chromogranin-A, Pancreastatin, Pituitary secretory protein I, Vasostatin I, SP-I, Vasostatin II
|Pathways||Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling|
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Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
|Restrictions||For Research Use only|
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|Reconstitution||After adding water, will consist of PBS buffer with 2 % sucrose|
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
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