anti-Human Chromogranin A antibody for BioImaging

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivity
Human
704
201
187
130
119
50
12
12
7
6
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
469
233
22
7
1
1
Clonality
Monoclonal
Conjugate
Un-conjugated
26
22
18
18
17
17
16
16
16
16
16
16
16
16
16
15
13
8
2
1
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
509
395
386
327
219
213
174
155
117
31
13
13
7
4
3
2
2
1
Options
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Catalog No. ABIN2689472
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General

Antigen Chromogranin A (CHGA) Antibodies
Reactivity Human
(704), (201), (187), (130), (119), (50), (12), (12), (7), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(469), (233), (22), (7), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(26), (22), (18), (18), (17), (17), (16), (16), (16), (16), (16), (16), (16), (16), (16), (15), (13), (8), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application BioImaging (BI), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
(509), (395), (386), (327), (219), (213), (174), (155), (117), (31), (13), (13), (7), (4), (3), (2), (2), (1)
Pubmed 5 references available
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Product details

Target Details Application Details Handling References Images
Brand BD Pharmingen™
Characteristics Chromogranin A (CGA) is a member of the granin family of regulated secretory proteins that are found in secretory granules in endocrine and neuroendocrine cells and released in response to extracellular stimulation. Intracellularly, granins are important for targeting peptide hormones and neurotransmitters by their ability to aggregate in the low pH , high calcium environment of the trans-Golgi network. Extracellularly, peptides formed from proteolytic processing of granins regulate hormone secretion. CGA is a prohormone that can be cleaved into several biologically active peptides, such as pancreastatin, β-granin, vasostatin, catestatin, and parastatin. β-granin is an N-terminal fragment of CGA, while pancreastatin and catestatin are processed from the central region of CGA. Cells of the adrenal medulla, anterior pituitary, cerebral cortex as well as beta cells of the pancreas and a variety of tumor cell lines express CGA. The expression of CGA can be used to monitor the pancreatic differentiation of pluripotent stem cells. Flow cytometric analysis of Chromogranin A expression in human neuroblastoma cells. SH-SY5Y neuroblastoma cells (ATCC CRL-2266) were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714), washed twice with BD Perm/Wash™ Buffer (Cat. No. 554723) , then stained with either Purified Mouse Anti-Human Chromogranin A or Purified Mouse IgG1, κ Isotype Control (Cat. No.555746) monoclonal antibodies followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589) second-step reagent. The fixed cells were resuspended in stain buffer and acquired on a BD LSR™ II

BD Pharmingen™ Purified Mouse Anti-Human Chromogranin A - Purified - Clone S21-537 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Chromogranin A Recombinant Protein
Clone S21
Isotype IgG1, kappa

Target Details

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Antigen
Alternative Name Chromogranin A (CHGA Antibody Abstract)
Background Synonyms: CGA, CgA, CHGA, Chromogranin-A, CMGA, SP-I
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Flow Cytometry System. The dotted line shows isotype control background staining, while the solid line represents Chromogranin A staining. The overlay histograms were derived from gated events with forward and side light-scatter characteristics of viable cells. Additionally, Purified Mouse Anti-Human Chromogranin A was negative on human embryonic stem (ES) cells (data not shown). Immunofluorescent staining of Chromogranin A in human neuroblastoma cells. SH-SY5Y neuroblastoma cells (ATCC CRL-2266) were fixed with BD Cytofix™ Fixation Buffer , permeabilized with BD Perm/Wash™ Buffer and stained with Purified Mouse Anti-Human Chromogranin A monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter staining was with DAPI (pseudo-colored blue). No Chromogranin A staining was observed on human embryonic stem cells (hESC) that were imaged similarly (data not shown). Also, no staining was observed when SH-SY5Y and hESC were stained with second-step reagent alone (data not shown). The image was captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. BD Phosflow™ Perm Buffer III or Triton™ X-100 are also suitable for permeabilization. Western Blot analysis of Chromogranin A in human SH-SY5Y neuroblastoma lysate. SH-SY5Y neuroblastoma (ATCC CRL-2266) cell lysate was probed with Purified Mouse Anti-Human Chromogranin A monoclonal antibody at titrations of 4.0 to 0.007 μg/lane. Data shown in lanes 1,2,3 are two fold dilutions, 0.25 μg, 0.125 μg, 0.063 μg. Chromogranin A is identified as a band of ~70 kDa. 564562 Rev. 1 Page 1 of 2
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References

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Product cited in:

Loh, Cheng, Mahata, Corti, Tota: "Chromogranin A and derived peptides in health and disease." in: Journal of molecular neuroscience : MN, Vol. 48, Issue 2, pp. 347-56, 2012

Schulz, Young, Agulnick, Babin, Baetge, Bang, Bhoumik, Cepa, Cesario, Haakmeester, Kadoya, Kelly, Kerr, Martinson, McLean, Moorman, Payne, Richardson, Ross, Sherrer, Song, Wilson, Brandon, Green et al.: "A scalable system for production of functional pancreatic progenitors from human embryonic stem cells. ..." in: PLoS ONE, Vol. 7, Issue 5, pp. e37004, 2012

Kroon, Martinson, Kadoya, Bang, Kelly, Eliazer, Young, Richardson, Smart, Cunningham, Agulnick, DAmour, Carpenter, Baetge: "Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo." in: Nature biotechnology, Vol. 26, Issue 4, pp. 443-52, 2008

DAmour, Bang, Eliazer, Kelly, Agulnick, Smart, Moorman, Kroon, Carpenter, Baetge: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." in: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, 2006

Mouland, Bevan, White, Hendy: "Human chromogranin A gene. Molecular cloning, structural analysis, and neuroendocrine cell-specific expression." in: The Journal of biological chemistry, Vol. 269, Issue 9, pp. 6918-26, 1994