anti-Human Chromogranin A antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Chromogranin A Antibody (supplied by: Log in to see )

Antigen
Chromogranin A (CHGA) Antibodies
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivity
Human
999
316
258
206
167
123
12
11
6
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
684
316
22
7
1
1
Clonality
Monoclonal
Conjugate
APC
40
35
31
30
27
16
16
16
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
14
12
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
680
630
459
403
303
211
211
196
168
102
16
13
13
8
7
3
2
1
1
Supplier
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Catalog No. ABIN6160547
$ 392.62
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6160555 FACS IF IHC (f) PE Mouse IgG2b, kappa Log in to see CHGA-413 0
1 ABIN6160554 FACS IF IHC (f) PerCP Mouse IgG2b, kappa Log in to see CHGA-413 0
1 ABIN6160623 FACS IF IHC (f) WB APC Mouse IgG1, kappa Log in to see CHGA-777 0
1 ABIN6160630 FACS IF IHC (f) WB PerCP Mouse IgG1, kappa Log in to see CHGA-777 0
1 ABIN6160631 FACS IF IHC (f) WB PE Mouse IgG1, kappa Log in to see CHGA-777 0
1 ABIN6160661 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see CHGA-798 0
1 ABIN6160668 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see CHGA-798 0
1 ABIN6160669 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see CHGA-798 0
1 ABIN6160558 FACS IF IHC (f) Mouse IgG2b, kappa Log in to see CHGA-413 0
1 ABIN6160634 FACS IF IHC (f) WB Mouse IgG1, kappa Log in to see CHGA-777 0
1 ABIN6160672 FACS IF IHC (f) Mouse IgG1, kappa Log in to see CHGA-798 0
1 ABIN6160471 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see CGA-414 0
1 ABIN6160478 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see CGA-414 0
1 ABIN6160479 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see CGA-414 0
1 ABIN6160585 FACS IF IHC (f) APC Mouse IgG2a, kappa Log in to see CHGA-765 0
1 ABIN6160593 FACS IF IHC (f) PE Mouse IgG2a, kappa Log in to see CHGA-765 0
1 ABIN6160592 FACS IF IHC (f) PerCP Mouse IgG2a, kappa Log in to see CHGA-765 0
1 ABIN6160775 FACS IF IHC (f) APC Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160782 FACS IF IHC (f) PerCP Mouse IgG1, kappa Log in to see LK2H10 0
1 ABIN6160783 FACS IF IHC (f) PE Mouse IgG1, kappa Log in to see LK2H10 0

General

Antigen Chromogranin A (CHGA) Antibodies
Reactivity Human
(999), (316), (258), (206), (167), (123), (12), (11), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(684), (316), (22), (7), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate APC
(40), (35), (31), (30), (27), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(680), (630), (459), (403), (303), (211), (211), (196), (168), (102), (16), (13), (13), (8), (7), (3), (2), (1), (1)
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Product details

Target Details Application Details Handling Images
Purpose Mouse Monoclonal anti-Chromogranin A (CHGA/413), APC Conjugate
Characteristics Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant human chromogranin A protein
Clone CHGA-413
Isotype IgG2b, kappa

Target Details

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Antigen
Alternative Name Chromogranin A (CHGA Antibody Abstract)
Background Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II
Molecular Weight 68-75 kDa
Gene ID 1113, 150793
UniProt P10645
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

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Application Notes Does not react with rat, others not known
  • Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

PC12 cells. Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

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Supplier Images
 image for anti-Chromogranin A (CHGA) antibody (APC) (ABIN6160547) anti-Chromogranin A (CHGA) antibody (APC)
 image for anti-Chromogranin A (CHGA) antibody (APC) (ABIN6160547) anti-Chromogranin A (CHGA) antibody (APC) (Image 2)
 image for anti-Chromogranin A (CHGA) antibody (APC) (ABIN6160547) anti-Chromogranin A (CHGA) antibody (APC) (Image 3)
 image for anti-Chromogranin A (CHGA) antibody (APC) (ABIN6160547) anti-Chromogranin A (CHGA) antibody (APC) (Image 4)